Cytogenetic analysis of childhood endodermal sinus tumors: A pediatric oncology group study

Elizabeth J. Perlman; Barbara Cushing; Edith Hawkins; Constance A. Griffin

doi:10.3109/15513819409023342

Cytogenetic analysis of childhood endodermal sinus tumors: A pediatric oncology group study

Elizabeth J. Perlman^*, Barbara Cushing, Edith Hawkins, Constance A. Griffin

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Most adult germ cell tumors have a consistent cytogenetic abnormality, i(12p), and are aneuploid. Many pediatric germ cell tumors are biologically distinct from their adult counterparts, particularly endodermal sinus tumors (ESTs) of young children. We report cytogenetic and ploidy analysis of nine ESTs involving children under 3 years of age (four extragonadal and five testicular). Structural abnormalities were present in seven tumors and were identifiable in six: 5/6 had a structural abnormality of chromosome 1, usually terminal deletion of 1p; 5/6 showed 6q deletion; 3/6 had structural abnormalities of 3p; 2/6 showed abnormalities of chromosome 2. None showed an i(12p) or abnormality of chromosome 12. Ploidy analysis of the tumors correlated with the cytogenetic analysis; in particular, the tumor that was cytogenetically normal showed no aneuploid peaks. To determine if a marker chromosome was derived from chromosome 12 or if karyotypically normal cases included nondividing tumor cells, interphase fluorescence in situ hybridization using an alpha satellite probe for chromosome 12 was performed. These studies showed no evidence of an i(12p). We conclude that ESTs in young children show cytogenetic differences from their adult counterparts and that loci on 1p, 6q, and 3q need to be further studied..

Original language	English (US)
Pages (from-to)	695-708
Number of pages	14
Journal	Fetal and Pediatric Pathology
Volume	14
Issue number	4
DOIs	https://doi.org/10.3109/15513819409023342
State	Published - 1994

Funding

This study was supported in part by the Society for Pediatric Pathology Young Investigator Grant (EP), grant CA46289-03f rom the NIH-NCI (CG) and grant CA-30969f rom the National Cancer Institute. The authors acknowledge the pathologists and pediatric oncologistsw ho took the time and effort to submit the tumors now reported as well as those from older pediatric germ cell tumors that will be reported in the future. In particular, we thank those involved in tumor submission from Hopital Sainte-Justine; Children's Hospital of Birmingham, Alabama; East Carolina University School of Medicine; Miami Children's Hospital; Texas Children's Hospital; and The Johns Hopkins Hospital.

Keywords

Cytogenetics
Endodermal sinus tumor
Fluorescence in situ hybridization
Germ cell tumor

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Pathology and Forensic Medicine

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10.3109/15513819409023342

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@article{16f3306d8602418587f1de4e2523fd48,

title = "Cytogenetic analysis of childhood endodermal sinus tumors: A pediatric oncology group study",

abstract = "Most adult germ cell tumors have a consistent cytogenetic abnormality, i(12p), and are aneuploid. Many pediatric germ cell tumors are biologically distinct from their adult counterparts, particularly endodermal sinus tumors (ESTs) of young children. We report cytogenetic and ploidy analysis of nine ESTs involving children under 3 years of age (four extragonadal and five testicular). Structural abnormalities were present in seven tumors and were identifiable in six: 5/6 had a structural abnormality of chromosome 1, usually terminal deletion of 1p; 5/6 showed 6q deletion; 3/6 had structural abnormalities of 3p; 2/6 showed abnormalities of chromosome 2. None showed an i(12p) or abnormality of chromosome 12. Ploidy analysis of the tumors correlated with the cytogenetic analysis; in particular, the tumor that was cytogenetically normal showed no aneuploid peaks. To determine if a marker chromosome was derived from chromosome 12 or if karyotypically normal cases included nondividing tumor cells, interphase fluorescence in situ hybridization using an alpha satellite probe for chromosome 12 was performed. These studies showed no evidence of an i(12p). We conclude that ESTs in young children show cytogenetic differences from their adult counterparts and that loci on 1p, 6q, and 3q need to be further studied..",

keywords = "Cytogenetics, Endodermal sinus tumor, Fluorescence in situ hybridization, Germ cell tumor",

author = "Perlman, {Elizabeth J.} and Barbara Cushing and Edith Hawkins and Griffin, {Constance A.}",

note = "Funding Information: This study was supported in part by the Society for Pediatric Pathology Young Investigator Grant (EP), grant CA46289-03f rom the NIH-NCI (CG) and grant CA-30969f rom the National Cancer Institute. The authors acknowledge the pathologists and pediatric oncologistsw ho took the time and effort to submit the tumors now reported as well as those from older pediatric germ cell tumors that will be reported in the future. In particular, we thank those involved in tumor submission from Hopital Sainte-Justine; Children's Hospital of Birmingham, Alabama; East Carolina University School of Medicine; Miami Children's Hospital; Texas Children's Hospital; and The Johns Hopkins Hospital.",

year = "1994",

doi = "10.3109/15513819409023342",

language = "English (US)",

volume = "14",

pages = "695--708",

journal = "Fetal and Pediatric Pathology",

issn = "1551-3815",

publisher = "Informa Healthcare",

number = "4",

}

TY - JOUR

T1 - Cytogenetic analysis of childhood endodermal sinus tumors

T2 - A pediatric oncology group study

AU - Perlman, Elizabeth J.

AU - Cushing, Barbara

AU - Hawkins, Edith

AU - Griffin, Constance A.

N1 - Funding Information: This study was supported in part by the Society for Pediatric Pathology Young Investigator Grant (EP), grant CA46289-03f rom the NIH-NCI (CG) and grant CA-30969f rom the National Cancer Institute. The authors acknowledge the pathologists and pediatric oncologistsw ho took the time and effort to submit the tumors now reported as well as those from older pediatric germ cell tumors that will be reported in the future. In particular, we thank those involved in tumor submission from Hopital Sainte-Justine; Children's Hospital of Birmingham, Alabama; East Carolina University School of Medicine; Miami Children's Hospital; Texas Children's Hospital; and The Johns Hopkins Hospital.

PY - 1994

Y1 - 1994

N2 - Most adult germ cell tumors have a consistent cytogenetic abnormality, i(12p), and are aneuploid. Many pediatric germ cell tumors are biologically distinct from their adult counterparts, particularly endodermal sinus tumors (ESTs) of young children. We report cytogenetic and ploidy analysis of nine ESTs involving children under 3 years of age (four extragonadal and five testicular). Structural abnormalities were present in seven tumors and were identifiable in six: 5/6 had a structural abnormality of chromosome 1, usually terminal deletion of 1p; 5/6 showed 6q deletion; 3/6 had structural abnormalities of 3p; 2/6 showed abnormalities of chromosome 2. None showed an i(12p) or abnormality of chromosome 12. Ploidy analysis of the tumors correlated with the cytogenetic analysis; in particular, the tumor that was cytogenetically normal showed no aneuploid peaks. To determine if a marker chromosome was derived from chromosome 12 or if karyotypically normal cases included nondividing tumor cells, interphase fluorescence in situ hybridization using an alpha satellite probe for chromosome 12 was performed. These studies showed no evidence of an i(12p). We conclude that ESTs in young children show cytogenetic differences from their adult counterparts and that loci on 1p, 6q, and 3q need to be further studied..

AB - Most adult germ cell tumors have a consistent cytogenetic abnormality, i(12p), and are aneuploid. Many pediatric germ cell tumors are biologically distinct from their adult counterparts, particularly endodermal sinus tumors (ESTs) of young children. We report cytogenetic and ploidy analysis of nine ESTs involving children under 3 years of age (four extragonadal and five testicular). Structural abnormalities were present in seven tumors and were identifiable in six: 5/6 had a structural abnormality of chromosome 1, usually terminal deletion of 1p; 5/6 showed 6q deletion; 3/6 had structural abnormalities of 3p; 2/6 showed abnormalities of chromosome 2. None showed an i(12p) or abnormality of chromosome 12. Ploidy analysis of the tumors correlated with the cytogenetic analysis; in particular, the tumor that was cytogenetically normal showed no aneuploid peaks. To determine if a marker chromosome was derived from chromosome 12 or if karyotypically normal cases included nondividing tumor cells, interphase fluorescence in situ hybridization using an alpha satellite probe for chromosome 12 was performed. These studies showed no evidence of an i(12p). We conclude that ESTs in young children show cytogenetic differences from their adult counterparts and that loci on 1p, 6q, and 3q need to be further studied..

KW - Cytogenetics

KW - Endodermal sinus tumor

KW - Fluorescence in situ hybridization

KW - Germ cell tumor

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JO - Fetal and Pediatric Pathology

JF - Fetal and Pediatric Pathology

IS - 4

ER -

Cytogenetic analysis of childhood endodermal sinus tumors: A pediatric oncology group study

Abstract

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Keywords

ASJC Scopus subject areas

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