Cytokine and adhesion molecule requirements for lung injury induced by anti-glomerular basement membrane antibody

Michael S. Mulligan*, Alex B. Lentsch, Thomas P. Shanley, Masayuki Miyasaka, Kent J. Johnson, Peter A. Ward

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Acute hemorrhagic lung injury occurs in humans with anti-GBM antibody (Goodpasture's syndrome), however, the mechanism of this injury is still largely unknown. To date, treatment has been confined to steroids and plasmaphoresis. Infusion of anti-GBM antibody into rats caused lung injury with intra-alveolar hemorrhage and intrapulmonary accumulation of neutrophils. Lung injury was dependent on the presence of neutrophils and complement and required both TNFα and IL-1. Experiments employing blocking antibodies to adhesion molecules demonstrated requirements for the β1 integrin VLA-4, β2 integrins LFA-1 and Mac-1, and L-selectin. The endothelial cell adhesion molecules, E-selectin and ICAM-1, were also required for the full development of lung injury. Inhibition of TNFα or IL-1 or adhesion molecules reduced both lung injury and tissue neutrophil accumulation. Thus, this study underscores cytokine and adhesion molecule requirements for neutrophil mediated injury in lung and kidney caused by anti-GBM, suggesting potential targets for the treatment of Goodpasture's syndrome in humans.

Original languageEnglish (US)
Pages (from-to)403-417
Number of pages15
JournalInflammation
Volume22
Issue number4
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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