TY - JOUR
T1 - Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue
AU - Broderick, Gordon
AU - Katz, Ben Z.
AU - Fernandes, Henrique
AU - Fletcher, Mary A.
AU - Klimas, Nancy
AU - Smith, Frederick A.
AU - O'Gorman, Maurice R.G.
AU - Vernon, Suzanne D.
AU - Taylor, Renee
N1 - Funding Information:
This analysis was funded by the CFIDS Association of America grants to G Broderick, BZ Katz and NG Klimas; cohort recruitment and assessment was supported by grants from the US National Institute of Health, including R01 HD043301-05 (PI R Taylor), R21AA016635 (PI M.A. Fletcher) and R01AI065723 (PI MA Fletcher); and R01AI065723 (PI MA Fletcher); the US Department of Veterans Affairs, Merit Award to NG Klimas.
PY - 2012/9/13
Y1 - 2012/9/13
N2 - Background: As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.Methods: Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.Results: Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.Conclusion: These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.
AB - Background: As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.Methods: Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.Results: Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.Conclusion: These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.
KW - Chronic fatigue
KW - Classification model
KW - Cytokines
KW - Infectious mononucleosis
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U2 - 10.1186/1479-5876-10-191
DO - 10.1186/1479-5876-10-191
M3 - Article
C2 - 22973830
AN - SCOPUS:84866064369
SN - 1479-5876
VL - 10
JO - Journal of Translational Medicine
JF - Journal of Translational Medicine
IS - 1
M1 - 191
ER -