TY - JOUR
T1 - Cytokine gene expression in human lung transplant recipients
AU - Whitehead, B. F.
AU - Stoehr, C.
AU - Wu, C. J.
AU - Patterson, G.
AU - Burchard, E. G.
AU - Theodore, J.
AU - Clayberger, C.
AU - Starnes, V. A.
PY - 1993
Y1 - 1993
N2 - The polymerase chain reaction was used to evaluate cytokine gene expression in bronchoalveolar lavage (BAL) cells and peripheral blood leukocytes in 31 human lung transplant recipients. All patients were maintained on a triple immunosuppression regimen consisting of CsA, AZA, and prednisone. Posttransplant survival ranged from 0.5 to 100.5 months (mean = 16.3 months). Cytokines IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, TNF-β, and IFN-γ were studied. In BAL, transcripts for IL-1α, IL-7, IL-8, and TNF- β were found in over 60% of samples and those for IL-5, IL-6, and IFN-γ in 40-50%, while IL-2 and IL-4 mRNA were rarely found (<20%). Considerable variation in the frequency of cytokine gene expression between BAL and peripheral blood was observed. When analyzed for the presence of acute pulmonary allograft rejection (without infection), transcripts for IL-4 and IL-6 in BAL demonstrated the greatest increase in frequency compared with nil rejection (P=0.07 and P=0.17, respectively). Pulmonary infection (without rejection) was associated with a modest increase in the expression of genes for IL-1α and IFN-γ (>10%). Transcripts for IL-4 were not found in association with pulmonary infection, suggesting that this cytokine may be useful as a discriminatory rejection marker.
AB - The polymerase chain reaction was used to evaluate cytokine gene expression in bronchoalveolar lavage (BAL) cells and peripheral blood leukocytes in 31 human lung transplant recipients. All patients were maintained on a triple immunosuppression regimen consisting of CsA, AZA, and prednisone. Posttransplant survival ranged from 0.5 to 100.5 months (mean = 16.3 months). Cytokines IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, TNF-β, and IFN-γ were studied. In BAL, transcripts for IL-1α, IL-7, IL-8, and TNF- β were found in over 60% of samples and those for IL-5, IL-6, and IFN-γ in 40-50%, while IL-2 and IL-4 mRNA were rarely found (<20%). Considerable variation in the frequency of cytokine gene expression between BAL and peripheral blood was observed. When analyzed for the presence of acute pulmonary allograft rejection (without infection), transcripts for IL-4 and IL-6 in BAL demonstrated the greatest increase in frequency compared with nil rejection (P=0.07 and P=0.17, respectively). Pulmonary infection (without rejection) was associated with a modest increase in the expression of genes for IL-1α and IFN-γ (>10%). Transcripts for IL-4 were not found in association with pulmonary infection, suggesting that this cytokine may be useful as a discriminatory rejection marker.
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U2 - 10.1097/00007890-199310000-00034
DO - 10.1097/00007890-199310000-00034
M3 - Article
C2 - 7692639
AN - SCOPUS:0027376566
SN - 0041-1337
VL - 56
SP - 956
EP - 961
JO - Transplantation
JF - Transplantation
IS - 4
ER -