Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

Lavanya Joshi, Meenakshi Ponnana, Ramya Sivangala, Lakshmi Kiran Chelluri, Pratibha Nallari, Vijaya Lakshmi Valluri, Sumanlatha Gaddam*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Immunological Methods
Volume432
DOIs
StatePublished - May 1 2016

Fingerprint

Pulmonary Tuberculosis
Age Groups
Cytokines
Messenger RNA
Interleukin-6
Interleukin-10
Tuberculosis
Logistic Models
Regression Analysis
Antigens
Infection

Keywords

  • Cytokines
  • Household contacts
  • Mycobacterium tuberculosis
  • r32kd M. bovis BCG

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Joshi, Lavanya ; Ponnana, Meenakshi ; Sivangala, Ramya ; Chelluri, Lakshmi Kiran ; Nallari, Pratibha ; Valluri, Vijaya Lakshmi ; Gaddam, Sumanlatha. / Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years). In: Journal of Immunological Methods. 2016 ; Vol. 432. pp. 65-71.
@article{63375774f63541db80812a121486911c,
title = "Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)",
abstract = "Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.",
keywords = "Cytokines, Household contacts, Mycobacterium tuberculosis, r32kd M. bovis BCG",
author = "Lavanya Joshi and Meenakshi Ponnana and Ramya Sivangala and Chelluri, {Lakshmi Kiran} and Pratibha Nallari and Valluri, {Vijaya Lakshmi} and Sumanlatha Gaddam",
year = "2016",
month = "5",
day = "1",
doi = "10.1016/j.jim.2016.02.012",
language = "English (US)",
volume = "432",
pages = "65--71",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",

}

Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years). / Joshi, Lavanya; Ponnana, Meenakshi; Sivangala, Ramya; Chelluri, Lakshmi Kiran; Nallari, Pratibha; Valluri, Vijaya Lakshmi; Gaddam, Sumanlatha.

In: Journal of Immunological Methods, Vol. 432, 01.05.2016, p. 65-71.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

AU - Joshi, Lavanya

AU - Ponnana, Meenakshi

AU - Sivangala, Ramya

AU - Chelluri, Lakshmi Kiran

AU - Nallari, Pratibha

AU - Valluri, Vijaya Lakshmi

AU - Gaddam, Sumanlatha

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

AB - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

KW - Cytokines

KW - Household contacts

KW - Mycobacterium tuberculosis

KW - r32kd M. bovis BCG

UR - http://www.scopus.com/inward/record.url?scp=84962157967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962157967&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2016.02.012

DO - 10.1016/j.jim.2016.02.012

M3 - Article

C2 - 26876300

AN - SCOPUS:84962157967

VL - 432

SP - 65

EP - 71

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -