Abstract
Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.
Original language | English (US) |
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Pages (from-to) | 65-71 |
Number of pages | 7 |
Journal | Journal of Immunological Methods |
Volume | 432 |
DOIs | |
State | Published - May 1 2016 |
Funding
We thank the staff of the Free Chest Clinic Mahavir PPMDOTS, Tuberculosis Unit (T.U.), Bhagwan Mahavir Trust. Financial support was provided by DBT-RGYI (Sanction no: 102/IFD/PR/2029/2007-2008 dated 18/01/2008) and COE (Sanction No: BT/01/COE/07/02 , dated 30/12/08).
Keywords
- Cytokines
- Household contacts
- Mycobacterium tuberculosis
- r32kd M. bovis BCG
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology