Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

Lavanya Joshi, Meenakshi Ponnana, Ramya Sivangala, Lakshmi Kiran Chelluri, Pratibha Nallari, Vijaya Lakshmi Valluri, Sumanlatha Gaddam*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Immunological Methods
Volume432
DOIs
StatePublished - May 1 2016

Keywords

  • Cytokines
  • Household contacts
  • Mycobacterium tuberculosis
  • r32kd M. bovis BCG

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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