TY - JOUR
T1 - Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)
AU - Joshi, Lavanya
AU - Ponnana, Meenakshi
AU - Sivangala, Ramya
AU - Chelluri, Lakshmi Kiran
AU - Nallari, Pratibha
AU - Valluri, Vijaya Lakshmi
AU - Gaddam, Sumanlatha
N1 - Funding Information:
We thank the staff of the Free Chest Clinic Mahavir PPMDOTS, Tuberculosis Unit (T.U.), Bhagwan Mahavir Trust. Financial support was provided by DBT-RGYI (Sanction no: 102/IFD/PR/2029/2007-2008 dated 18/01/2008) and COE (Sanction No: BT/01/COE/07/02 , dated 30/12/08).
Publisher Copyright:
© 2016.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.
AB - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.
KW - Cytokines
KW - Household contacts
KW - Mycobacterium tuberculosis
KW - r32kd M. bovis BCG
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U2 - 10.1016/j.jim.2016.02.012
DO - 10.1016/j.jim.2016.02.012
M3 - Article
C2 - 26876300
AN - SCOPUS:84962157967
VL - 432
SP - 65
EP - 71
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
ER -
