Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

Lavanya Joshi; Meenakshi Ponnana; Ramya Sivangala; Lakshmi Kiran Chelluri; Pratibha Nallari; Vijaya Lakshmi Valluri; Sumanlatha Gaddam

doi:10.1016/j.jim.2016.02.012

Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

Lavanya Joshi, Meenakshi Ponnana, Ramya Sivangala, Lakshmi Kiran Chelluri, Pratibha Nallari, Vijaya Lakshmi Valluri, Sumanlatha Gaddam^*

^*Corresponding author for this work

Medicine, Hospital Medicine Division

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

Original language	English (US)
Pages (from-to)	65-71
Number of pages	7
Journal	Journal of Immunological Methods
Volume	432
DOIs	https://doi.org/10.1016/j.jim.2016.02.012
State	Published - May 1 2016

Funding

We thank the staff of the Free Chest Clinic Mahavir PPMDOTS, Tuberculosis Unit (T.U.), Bhagwan Mahavir Trust. Financial support was provided by DBT-RGYI (Sanction no: 102/IFD/PR/2029/2007-2008 dated 18/01/2008) and COE (Sanction No: BT/01/COE/07/02 , dated 30/12/08).

Keywords

Cytokines
Household contacts
Mycobacterium tuberculosis
r32kd M. bovis BCG

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jim.2016.02.012

Cite this

@article{63375774f63541db80812a121486911c,

title = "Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)",

abstract = "Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.",

keywords = "Cytokines, Household contacts, Mycobacterium tuberculosis, r32kd M. bovis BCG",

author = "Lavanya Joshi and Meenakshi Ponnana and Ramya Sivangala and Chelluri, {Lakshmi Kiran} and Pratibha Nallari and Valluri, {Vijaya Lakshmi} and Sumanlatha Gaddam",

note = "Funding Information: We thank the staff of the Free Chest Clinic Mahavir PPMDOTS, Tuberculosis Unit (T.U.), Bhagwan Mahavir Trust. Financial support was provided by DBT-RGYI (Sanction no: 102/IFD/PR/2029/2007-2008 dated 18/01/2008) and COE (Sanction No: BT/01/COE/07/02 , dated 30/12/08). Publisher Copyright: {\textcopyright} 2016.",

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doi = "10.1016/j.jim.2016.02.012",

language = "English (US)",

volume = "432",

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T1 - Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

AU - Joshi, Lavanya

AU - Ponnana, Meenakshi

AU - Sivangala, Ramya

AU - Chelluri, Lakshmi Kiran

AU - Nallari, Pratibha

AU - Valluri, Vijaya Lakshmi

AU - Gaddam, Sumanlatha

N1 - Funding Information: We thank the staff of the Free Chest Clinic Mahavir PPMDOTS, Tuberculosis Unit (T.U.), Bhagwan Mahavir Trust. Financial support was provided by DBT-RGYI (Sanction no: 102/IFD/PR/2029/2007-2008 dated 18/01/2008) and COE (Sanction No: BT/01/COE/07/02 , dated 30/12/08). Publisher Copyright: © 2016.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

AB - Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p < 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.

KW - Cytokines

KW - Household contacts

KW - Mycobacterium tuberculosis

KW - r32kd M. bovis BCG

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ER -

Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25 years)

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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