Abstract
Purpose The contralateral unaffected breast (CUB) of women with unilateral breast cancer provides a model for the study of breast tissue-based risk factors. Using random fine needle aspiration (rFNA), we have investigated hormonal and gene expression patterns related to atypia in the CUBs of newly diagnosed breast cancer patients. Methods 83 women underwent rFNA of the CUB. Cytologic analysis was performed using the Masood Score (MS), atypia was defined as MS > 14. RNA was extracted using 80% of the sample. The expression of 20 hormone related genes was quantified using Taqman Low Density Arrays. Statistical analysis was performed using 2-tailed t tests and linear regression. Results Cytological atypia was more frequent in multiparous women (P = 0.0392), and was not associated with any tumor-related features in the affected breast. Masood Score was higher with shorter interval since last pregnancy (R = 0.204, P = 0.0417), higher number of births (R = 0.369, P = 0.0006), and estrogen receptor (ER) negativity of the index cancer (R = −0.203, P = 0.065). Individual cytologic features were associated with aspects of parity. Specifically, anisonucleosis was correlated with shorter interval since last pregnancy (R = 0.318, P = 0.0201), higher number of births (R = 0.382, P = 0.0004), and ER status (R = −0.314, P = 0.0038). Eight estrogen-regulated genes were increased in atypical samples (P < 0.005), including TFF1, AGT, PDZK1, PGR, GREB1, PRLR, CAMK2B, and CCND1. Conclusions Cytologic atypia, and particularly anisonucleosis, is associated with recent and multiple births and ER negative status of the index tumor. Atypical samples showed increased expression of estrogen-related genes, consistent with the role of estrogen exposure in breast cancer development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 449-456 |
| Number of pages | 8 |
| Journal | Surgical Oncology |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2016 |
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Keywords
- Anisonucleosis
- Breast cancer risk
- Contralateral unaffected breast
- Cytologic atypia
- Gene expression
- Random fine needle aspiration
ASJC Scopus subject areas
- Surgery
- Oncology
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Cytologic atypia in the contralateral unaffected breast is related to parity and estrogen-related genes. / Monahan, Denise A.; Wang, Jun; Lee, Oukseub; Revesz, Elizabeth; Taft, Nancy; Ivancic, David; Hansen, Nora M.; Bethke, Kevin P.; Zalles, C.; Khan, Seema A.
In: Surgical Oncology, Vol. 25, No. 4, 01.12.2016, p. 449-456.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cytologic atypia in the contralateral unaffected breast is related to parity and estrogen-related genes
AU - Monahan, Denise A.
AU - Wang, Jun
AU - Lee, Oukseub
AU - Revesz, Elizabeth
AU - Taft, Nancy
AU - Ivancic, David
AU - Hansen, Nora M.
AU - Bethke, Kevin P.
AU - Zalles, C.
AU - Khan, Seema A.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Purpose The contralateral unaffected breast (CUB) of women with unilateral breast cancer provides a model for the study of breast tissue-based risk factors. Using random fine needle aspiration (rFNA), we have investigated hormonal and gene expression patterns related to atypia in the CUBs of newly diagnosed breast cancer patients. Methods 83 women underwent rFNA of the CUB. Cytologic analysis was performed using the Masood Score (MS), atypia was defined as MS > 14. RNA was extracted using 80% of the sample. The expression of 20 hormone related genes was quantified using Taqman Low Density Arrays. Statistical analysis was performed using 2-tailed t tests and linear regression. Results Cytological atypia was more frequent in multiparous women (P = 0.0392), and was not associated with any tumor-related features in the affected breast. Masood Score was higher with shorter interval since last pregnancy (R = 0.204, P = 0.0417), higher number of births (R = 0.369, P = 0.0006), and estrogen receptor (ER) negativity of the index cancer (R = −0.203, P = 0.065). Individual cytologic features were associated with aspects of parity. Specifically, anisonucleosis was correlated with shorter interval since last pregnancy (R = 0.318, P = 0.0201), higher number of births (R = 0.382, P = 0.0004), and ER status (R = −0.314, P = 0.0038). Eight estrogen-regulated genes were increased in atypical samples (P < 0.005), including TFF1, AGT, PDZK1, PGR, GREB1, PRLR, CAMK2B, and CCND1. Conclusions Cytologic atypia, and particularly anisonucleosis, is associated with recent and multiple births and ER negative status of the index tumor. Atypical samples showed increased expression of estrogen-related genes, consistent with the role of estrogen exposure in breast cancer development.
AB - Purpose The contralateral unaffected breast (CUB) of women with unilateral breast cancer provides a model for the study of breast tissue-based risk factors. Using random fine needle aspiration (rFNA), we have investigated hormonal and gene expression patterns related to atypia in the CUBs of newly diagnosed breast cancer patients. Methods 83 women underwent rFNA of the CUB. Cytologic analysis was performed using the Masood Score (MS), atypia was defined as MS > 14. RNA was extracted using 80% of the sample. The expression of 20 hormone related genes was quantified using Taqman Low Density Arrays. Statistical analysis was performed using 2-tailed t tests and linear regression. Results Cytological atypia was more frequent in multiparous women (P = 0.0392), and was not associated with any tumor-related features in the affected breast. Masood Score was higher with shorter interval since last pregnancy (R = 0.204, P = 0.0417), higher number of births (R = 0.369, P = 0.0006), and estrogen receptor (ER) negativity of the index cancer (R = −0.203, P = 0.065). Individual cytologic features were associated with aspects of parity. Specifically, anisonucleosis was correlated with shorter interval since last pregnancy (R = 0.318, P = 0.0201), higher number of births (R = 0.382, P = 0.0004), and ER status (R = −0.314, P = 0.0038). Eight estrogen-regulated genes were increased in atypical samples (P < 0.005), including TFF1, AGT, PDZK1, PGR, GREB1, PRLR, CAMK2B, and CCND1. Conclusions Cytologic atypia, and particularly anisonucleosis, is associated with recent and multiple births and ER negative status of the index tumor. Atypical samples showed increased expression of estrogen-related genes, consistent with the role of estrogen exposure in breast cancer development.
KW - Anisonucleosis
KW - Breast cancer risk
KW - Contralateral unaffected breast
KW - Cytologic atypia
KW - Gene expression
KW - Random fine needle aspiration
UR - http://www.scopus.com/inward/record.url?scp=84998953557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84998953557&partnerID=8YFLogxK
U2 - 10.1016/j.suronc.2015.12.001
DO - 10.1016/j.suronc.2015.12.001
M3 - Article
C2 - 26856771
AN - SCOPUS:84998953557
VL - 25
SP - 449
EP - 456
JO - Surgical Oncology
JF - Surgical Oncology
SN - 0960-7404
IS - 4
ER -