TY - JOUR
T1 - Cytometric, morphologic and enzymatic characterisation of haemocytes in Anodonta cygnea
AU - Soares-Da-Silva, I. M.
AU - Ribeiro, J.
AU - Valongo, C.
AU - Pinto, R.
AU - Vilanova, M.
AU - Bleher, R.
AU - Machado, J.
N1 - Funding Information:
We would like to thank Professor Doutor Lobo da Cunha and Professor Doutora Fernanda Russel-Pinto for their help in the microscopy techniques. This work was supported by a grant from ‘Junta Nacional de Investigação Cientı́fica e Tecnológica’, Portugal.
PY - 2002
Y1 - 2002
N2 - The haemocytes in bivalve mussels are involved in many processes such as lesion repair, shell repair, elimination of small particles and toxic substances. In Anodonta cygnea there are two categories of haemolymph cells, the granulocytes and hyalinocytes. Two groups of cells were identified by flow cytometry and morphological studies: one with larger size and granularity representing 75%, and another group of cells (25%) which were approximately half the size. The cytochemical reactions showed peroxidase activity in the larger cells and a weak prophenoloxidase activity in the smaller cells. These characteristics suggest that the most common haemocytes are granulocytes and hyalinocytes are less common. Enzymatic studies showed clear activities of few enzymes in different compartments of the mantle. Both haemocytes presented significant variations for α-manosidase and β-glucurosidase activities depending on the acid or alkaline pH. Almost all were sensitive to the pH changes, mainly the β-galactosidase in the haemolymph plasma. On the contrary, the same enzymatic analysis in the extrapallial elements showed more stabilised activities. The simulation of acidic and alkaline condition with the observation of significant morphological and enzymatic activity changes, allow us to speculate some functional role, mainly in the haemolymph elements. The granulocytes may be speculated to have intense involvement in the digestion of small residues with the formation of calcareous stores while the hyalinocytes are more responsible for the elimination of soluble cytotoxic compounds.
AB - The haemocytes in bivalve mussels are involved in many processes such as lesion repair, shell repair, elimination of small particles and toxic substances. In Anodonta cygnea there are two categories of haemolymph cells, the granulocytes and hyalinocytes. Two groups of cells were identified by flow cytometry and morphological studies: one with larger size and granularity representing 75%, and another group of cells (25%) which were approximately half the size. The cytochemical reactions showed peroxidase activity in the larger cells and a weak prophenoloxidase activity in the smaller cells. These characteristics suggest that the most common haemocytes are granulocytes and hyalinocytes are less common. Enzymatic studies showed clear activities of few enzymes in different compartments of the mantle. Both haemocytes presented significant variations for α-manosidase and β-glucurosidase activities depending on the acid or alkaline pH. Almost all were sensitive to the pH changes, mainly the β-galactosidase in the haemolymph plasma. On the contrary, the same enzymatic analysis in the extrapallial elements showed more stabilised activities. The simulation of acidic and alkaline condition with the observation of significant morphological and enzymatic activity changes, allow us to speculate some functional role, mainly in the haemolymph elements. The granulocytes may be speculated to have intense involvement in the digestion of small residues with the formation of calcareous stores while the hyalinocytes are more responsible for the elimination of soluble cytotoxic compounds.
KW - Bivalve
KW - Calcareous concretions
KW - Enzymatic activity
KW - Flow cytometry
KW - Haemocytes
KW - Morphology
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U2 - 10.1016/S1095-6433(02)00039-9
DO - 10.1016/S1095-6433(02)00039-9
M3 - Article
C2 - 12044764
AN - SCOPUS:0035989449
VL - 132
SP - 541
EP - 553
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
SN - 1095-6433
IS - 3
ER -