Cytoplasmic movement profiles of mouse surrounding nucleolus and not-surrounding nucleolus antral oocytes during meiotic resumption

Thi Thu Hien Bui, Martina Belli, Lorenzo Fassina, Giulia Vigone, Valeria Merico*, Silvia Garagna, Maurizio Zuccotti

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Full-grown mouse antral oocytes are classified as surrounding nucleolus (SN) or not-surrounding nucleolus (NSN), depending on the respective presence or absence of a ring of Hoechst-positive chromatin surrounding the nucleolus. In culture, both types of oocytes resume meiosis and reach the metaphase II (MII) stage, but following insemination, NSN oocytes arrest at the two-cell stage whereas SN oocytes may develop to term. By coupling time-lapse bright-field microscopy with image analysis based on particle image velocimetry, we provide the first systematic measure of the changes to the cytoplasmic movement velocity (CMV) occurring during the germinal vesicle-to-MII (GV-to-MII) transition of these two types of oocytes. Compared to SN oocytes, NSN oocytes display a delayed GV-to-MII transition, which can be mostly explained by retarded germinal vesicle break down and first polar body extrusion. SN and NSN oocytes also exhibit significantly different CMV profiles at four main time-lapse intervals, although this difference was not predictive of SN or NSN oocyte origin because of the high variability in CMV. When CMV profile was analyzed through a trained artificial neural network, however, each single SN or NSN oocyte was blindly identified with a probability of 92.2% and 88.7%, respectively. Thus, the CMV profile recorded during meiotic resumption may be exploited as a cytological signature for the non-invasive assessment of the oocyte developmental potential, and could be informative for the analysis of the GV-to-MII transition of oocytes of other species.

Original languageEnglish (US)
Pages (from-to)356-362
Number of pages7
JournalMolecular reproduction and development
Volume84
Issue number5
DOIs
StatePublished - May 2017
Externally publishedYes

Keywords

  • cytoplasmic movement
  • feedforward artificial neural network
  • oocyte developmental competence
  • particle image velocimetry
  • time-lapse

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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