Cytoskeleton of human mononuclear cells as a possible peripheral marker for phenylalanine neurotoxicity in PKU

Sabrina Dick, Claáudia Funchal, Priscila De Lima Pelaez, Samanta Oliveira Loureiro, Lilian Vivian, Franciele Dall Bello Pessutto, Luúcia Maria Almeida, Clóvis Milton Duval Wannmacher, Regina Pessoa-Pureur

Research output: Contribution to journalArticlepeer-review

Abstract

In this work we tested human mononuclear cells as a peripheral marker to study neurotoxicity of phenylalanine (Phe). Slices of cerebral cortex of rats or human mononuclear cells were incubated with different concentrations of Phe and/or Ala in the presence of 32P-orthophosphate, the cytoskeletal fraction was extracted, and the radioactivity incorporated into intermediate filament proteins was measured. Our results show that 2 mM Phe as well as 1 mM Ala are effective in increasing the 32P in vitro incorporation into IFs in both tissues. When cerebral cortex slices or mononuclear cells were incubated with different concentrations of Phe and/or Ala, the effects on the 32P in vitro incorporation into IF proteins was compatible with an antagonistic mechanism of action of the two amino acids on the enzymes of the phosphorylating system. In addition, these blood cells may be a possible peripheral marker to study neurotoxicity of Phe in patients with PKU.

Original languageEnglish (US)
Article number455354
Pages (from-to)1569-1576
Number of pages8
JournalNeurochemical Research
Volume27
Issue number12
DOIs
StatePublished - 2002

Keywords

  • Alanine
  • Cytoskeleton
  • Intermediate filaments
  • Mononuclear cells
  • Phenylalanine
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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