We have studied the distribution of cytoskeletal elements in detergent-extracted mouse embryo fibroblasts using the platinum replica technique. It was shown that lamelloplasm can be subdivided into three zones: the ruffle edge with dense microfilaments meshwork; the sparse zone adjacent to the ruffle edge and containing relatively few cytoskeletal elements; the lamella proper occupied with a three-dimensional network of microfilaments, micotubules, intermediate filaments; this zone contained adhesion plaques corresponding to cell-substrate focal contacts and associated with the microfilament bundle ends. The cytoskeleton structure of the central (endoplasm) region of the cell was markedly different from that of the lamelloplasm. Its main feature was a dense microfilament sheath at the dorsal cell surface. Sites of microfilament bundle convergence can be visualized near the nucleus after partial removal of the sheath by more complete detergent extraction.
|Original language||English (US)|
|Number of pages||11|
|Journal||European Journal of Cell Biology|
|State||Published - Dec 1 1984|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology