D1-D2 complex of the photosystem II reaction center from spinach Isolation and partial characterization

Xiao Song Tang, Kazuo Fushimi, Kimiyuki Satoh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


A pigment-protein complex consisting of D1 and D2 proteins, but depleted in the two lower molecular mass components of photosystem II, i.e. cytochrome b-559 and psbI gene product, has been isolated by octyl-β-D-glucopyranoside treatment of the purified photosystem II reaction center complex from spinach [(1987) Proc. Natl. Acad. Sci. USA 84, 109-112], followed by separation by high performance liquid chromatography using a gel-permeation column (TSK G3000 SW). The isolated complex is photochemically active in the photoreduction of intrinsic pheophytin a under steady-state illumination, in the presence of dithionite and methyl viologen, and exhibits pigment stoichiometries similar to those in the untreated reaction center, indicating that the D1-D2 complex provides the site of primary photochemistry in photosystem II, as well as the principal binding sites of pigments in the reaction center.

Original languageEnglish (US)
Pages (from-to)257-260
Number of pages4
JournalFEBS Letters
Issue number1-2
StatePublished - Oct 29 1990


  • Charge separation
  • Cytochrome b-559
  • D1-D2 complex
  • Octyl-β-D-glucopyranoside
  • Photosystem II reaction center

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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