DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation

Jessica H. Spahn, Wenjun Li, Alejandro C. Bribriesco, Jie Liu, Hua Shen, Aida Richardson, Jie Hong Pan, Bernd H. Zinselmeyer, Steven L. Brody, Daniel R. Goldstein, Alexander S. Krupnick, Andrew E. Gelman, Mark J. Miller, Daniel Kreisel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Neutrophils are criticalmediators of innate immune responses and contribute to tissue injury. However, immune pathways that regulate neutrophil recruitment to injured tissues during noninfectious inflammation remain poorly understood. DAP12 is a cell membrane-associated protein that is expressed in myeloid cells and can either augment or dampen innate inflammatory responses during infections. To elucidate the role of DAP12 in pulmonary ischemia/reperfusion injury (IRI), we took advantage of a clinically relevant mouse model of transplant-mediated lung IRI. This technique allowed us to dissect the importance of DAP12 in tissue-resident cells and those that infiltrate injured tissue from the periphery during noninfectious inflammation.Macrophages in both mouse and human lungs that have been subjected to cold ischemic storage express DAP12. We found that donor, but not recipient, deficiency in DAP12 protected against pulmonary IRI. Analysis of the immune response showed that DAP12 promotes the survival of tissue-resident alveolar macrophages and contributes to local production of neutrophil chemoattractants. Intravital imaging demonstrated a transendothelial migration defect into DAP12-deficient lungs, which can be rescued by local administration of the neutrophil chemokine CXCL2.We have uncovered a previously unrecognized role for DAP12 expression in tissue-resident alveolar macrophages in mediating acute noninfectious tissue injury through regulation of neutrophil trafficking.

Original languageEnglish (US)
Pages (from-to)4039-4048
Number of pages10
JournalJournal of Immunology
Issue number8
StatePublished - Apr 15 2015

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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