Dark field X-ray microscopy: The effects of condenser/detector aperture

S. Vogt, H. N. Chapman, C. Jacobsen*, R. Medenwaldt

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In order to visualize the functionality of a biological cell, it is often desirable to label specific proteins. In this work we concentrate on the optical theory of visualizing colloidal gold labels with soft X-ray microscopes, where scattering from small gold spheres used as labels dominates the image. Using numerical simulations of bright and dark field imaging, we compare different configurations of condenser and objective lenses in transmission X-ray microscopes, and configurations of detector and objective lens in scanning transmission X-ray microscopes. It is verified that the contrast of small, closely spaced features is strongly affected by changes in these configurations; the optimum situation is to have the condenser aperture (in TXM) or detector aperture (in STXM) equal to 32 that of the objective numerical aperture.

Original languageEnglish (US)
Pages (from-to)25-44
Number of pages20
JournalUltramicroscopy
Volume87
Issue number1-2
DOIs
StatePublished - Mar 28 2001

Keywords

  • Dark field
  • Labeling
  • X-ray microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

Fingerprint Dive into the research topics of 'Dark field X-ray microscopy: The effects of condenser/detector aperture'. Together they form a unique fingerprint.

  • Cite this