De novo design of signal sequences to localize cargo to the 1,2-propanediol utilization microcompartment

Christopher M. Jakobson, Marilyn F. Slininger Lee, Danielle Tullman-Ercek*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Organizing heterologous biosyntheses inside bacterial cells can alleviate common problems owing to toxicity, poor kinetic performance, and cofactor imbalances. A subcellular organelle known as a bacterial microcompartment, such as the 1,2-propanediol utilization microcompartment of Salmonella, is a promising chassis for this strategy. Here we demonstrate de novo design of the N-terminal signal sequences used to direct cargo to these microcompartment organelles. We expand the native repertoire of signal sequences using rational and library-based approaches and show that a canonical leucine-zipper motif can function as a signal sequence for microcompartment localization. Our strategy can be applied to generate new signal sequences localizing arbitrary cargo proteins to the 1,2-propanediol utilization microcompartments.

Original languageEnglish (US)
Pages (from-to)1086-1092
Number of pages7
JournalProtein Science
Volume26
Issue number5
DOIs
StatePublished - May 1 2017

Keywords

  • Salmonella
  • bacterial microcompartments
  • nanoreactor
  • signal sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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