Abstract
Organizing heterologous biosyntheses inside bacterial cells can alleviate common problems owing to toxicity, poor kinetic performance, and cofactor imbalances. A subcellular organelle known as a bacterial microcompartment, such as the 1,2-propanediol utilization microcompartment of Salmonella, is a promising chassis for this strategy. Here we demonstrate de novo design of the N-terminal signal sequences used to direct cargo to these microcompartment organelles. We expand the native repertoire of signal sequences using rational and library-based approaches and show that a canonical leucine-zipper motif can function as a signal sequence for microcompartment localization. Our strategy can be applied to generate new signal sequences localizing arbitrary cargo proteins to the 1,2-propanediol utilization microcompartments.
Original language | English (US) |
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Pages (from-to) | 1086-1092 |
Number of pages | 7 |
Journal | Protein Science |
Volume | 26 |
Issue number | 5 |
DOIs | |
State | Published - May 1 2017 |
Keywords
- Salmonella
- bacterial microcompartments
- nanoreactor
- signal sequence
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry