Decoding protein modifications using top-down mass spectrometry

Nertila Siuti*, Neil L. Kelleher

*Corresponding author for this work

Research output: Contribution to journalArticle

345 Scopus citations

Abstract

Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.

Original languageEnglish (US)
Pages (from-to)817-821
Number of pages5
JournalNature Methods
Volume4
Issue number10
DOIs
StatePublished - Oct 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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