Deconvolution of the Cellular Oxidative Stress Response with Organelle-Specific Peptide Conjugates

Kerry P. Mahon, Terra B. Potocky, Derek Blair, Marc D. Roy, Kelly M. Stewart, Thomas C. Chiles, Shana O. Kelley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Oxidative stress is a deleterious force that must be combated relentlessly by aerobic organisms and is known to underlie many human diseases including atherosclerosis, Parkinson's disease, and Alzheimer's disease. Information available about the oxidative stress response has come primarily from studies using reactive oxygen species (ROS) with ill-defined locations within the cell. Thus, existing models do not account for possible differences between stress originating within particular regions of the cell. Here, oxidative stress is studied at the subcellular level using ROS-generating compounds localizing within two different organelles: the nucleus and the mitochondrion. Differences in cytotoxicity, gene expression, and survival pathway activation are detected as a function of the subcellular origin of oxidative stress, indicating that independent mechanisms are used to cope with oxidative stress arising in different cellular compartments. These comparative studies, enabled by the development of organelle-specific oxidants, examine the cellular responses to site-specific oxidative stress with heightened precision.

Original languageEnglish (US)
Pages (from-to)923-930
Number of pages8
JournalChemistry and Biology
Volume14
Issue number8
DOIs
StatePublished - Aug 24 2007
Externally publishedYes

Keywords

  • CHEMBIO
  • SIGNALING

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Deconvolution of the Cellular Oxidative Stress Response with Organelle-Specific Peptide Conjugates'. Together they form a unique fingerprint.

Cite this