Decreased de novo synthesis of glomerular proteoglycans in diabetes: Biochemical and autoradiographic evidence

Y. S. Kanwar, L. J. Rosenzweig, A. Linker, M. L. Jakubowski

Research output: Contribution to journalArticlepeer-review

144 Scopus citations

Abstract

The experimental model of streptozotocin-induced diabetes in rats was utilized to determine the biosynthetic and biochemical alterations in the proteoglycans of the glomerular extracellular matrices (glomerular basement membrane and mesangial matrix) in diabetic nephropathy. Isolated kidneys from diabetic and control groups of animals were radiolabeled in an organ perfusion apparatus with [35S]sulfate of high specific activity (>1,200 Ci/mmol; 1 Ci = 3.7 x 1010 Bq) and processed for electron microscopic autoradiography, and the proteoglycans of the glomerular extracelluar matrices were characterized. The results indicate that [35S]sulfate incorporation into glomerular extracellular matrices of diabetic animals was 30-40% less than that of the control group; however, no differences in the biochemical properties of the de novo synthesized proteoglycans from either group were observed. The relevance of the decreased de novo synthesis of sulfated proteoglycans of glomerular extracellular matrices is discussed in terms of increased glomerular permeability to plasma proteins and reduction in the glomerular filtration rate.

Original languageEnglish (US)
Pages (from-to)2272-2275
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number8 I
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Decreased de novo synthesis of glomerular proteoglycans in diabetes: Biochemical and autoradiographic evidence'. Together they form a unique fingerprint.

Cite this