Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf

Lingyan Shi; Adrián Rodríguez-Contreras; Yury Budansky; Yang Pu; Thien An Nguyen; Robert R. Alfano

doi:10.1117/1.JBO.19.6.066009

Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf

Lingyan Shi, Adrián Rodríguez-Contreras, Yury Budansky, Yang Pu, Thien An Nguyen, Robert R. Alfano^*

^*Corresponding author for this work

Communication Sciences and Disorders

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Two-photon (2P) excitation of the second singlet (S₂) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the "tissue optical window" (650 to 950 nm). S₂ state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S₂ state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.

Original language	English (US)
Article number	066009
Journal	Journal of Biomedical Optics
Volume	19
Issue number	6
DOIs	https://doi.org/10.1117/1.JBO.19.6.066009
State	Published - Jun 2014

Funding

We thank Professor Bingmei Fu for providing partial support of Lingyan Shi. This research is supported in part by the Corning grant, Airforce Research Office (ARO), and City College internal funds to Robert Alfano. This research is also supported by Research Enhancement Award 5SC1HD068129 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development to Dr. Adrián Rodríguez-Contreras, and grants from the National Center for Research Resources (2G12RR03060-26A1) and the National Institute on Minority Health Disparities (8G12MD007603-27) from the National Institutes of Health.

Keywords

chlorophyll α
penetration depth
second singlet excitation
tissue optical window
two photon

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.JBO.19.6.066009

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title = "Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf",

abstract = "Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the {"}tissue optical window{"} (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.",

keywords = "chlorophyll α, penetration depth, second singlet excitation, tissue optical window, two photon",

author = "Lingyan Shi and Adri{\'a}n Rodr{\'i}guez-Contreras and Yury Budansky and Yang Pu and {An Nguyen}, Thien and Alfano, {Robert R.}",

note = "Funding Information: We thank Professor Bingmei Fu for providing partial support of Lingyan Shi. This research is supported in part by the Corning grant, Airforce Research Office (ARO), and City College internal funds to Robert Alfano. This research is also supported by Research Enhancement Award 5SC1HD068129 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development to Dr. Adri{\'a}n Rodr{\'i}guez-Contreras, and grants from the National Center for Research Resources (2G12RR03060-26A1) and the National Institute on Minority Health Disparities (8G12MD007603-27) from the National Institutes of Health.",

year = "2014",

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doi = "10.1117/1.JBO.19.6.066009",

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AU - Shi, Lingyan

AU - Rodríguez-Contreras, Adrián

AU - Budansky, Yury

AU - Pu, Yang

AU - An Nguyen, Thien

AU - Alfano, Robert R.

N1 - Funding Information: We thank Professor Bingmei Fu for providing partial support of Lingyan Shi. This research is supported in part by the Corning grant, Airforce Research Office (ARO), and City College internal funds to Robert Alfano. This research is also supported by Research Enhancement Award 5SC1HD068129 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development to Dr. Adrián Rodríguez-Contreras, and grants from the National Center for Research Resources (2G12RR03060-26A1) and the National Institute on Minority Health Disparities (8G12MD007603-27) from the National Institutes of Health.

PY - 2014/6

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N2 - Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the "tissue optical window" (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.

AB - Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the "tissue optical window" (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.

KW - chlorophyll α

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KW - tissue optical window

KW - two photon

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Deep two-photon microscopic imaging through brain tissue using the second singlet state from fluorescent agent chlorophyll α in spinach leaf

Abstract

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Keywords

ASJC Scopus subject areas

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