Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum

Harry P. Bassaris, Popi E. Lianou, Athanasius T. Skoutelis, John T. Papavassiliou, John Phillip Phair, Harry P. Bassaris, Popi E. Lianou, Athanasius T. Skoutelis, John T. Papavassiliou, John P. Phair

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of sera obtained from 18 patients with homozygous f3-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1010 ± 2.5010 (mean ± sn) compared with 17.9010 ± 8.0010 adherence in control serum (P< 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6010 ± 6.2010 (P < 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.

Original languageEnglish (US)
Pages (from-to)52-55
Number of pages4
JournalJournal of Infectious Diseases
Volume146
Issue number1
DOIs
StatePublished - Jan 1 1982

Fingerprint

Nylons
Neutrophils
Serum
Zymosan
Thalassemia
Chemotaxis
Chemotactic Factors
Hot Temperature

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Bassaris, H. P., Lianou, P. E., Skoutelis, A. T., Papavassiliou, J. T., Phair, J. P., Bassaris, H. P., ... Phair, J. P. (1982). Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum. Journal of Infectious Diseases, 146(1), 52-55. https://doi.org/10.1093/infdis/146.1.52
Bassaris, Harry P. ; Lianou, Popi E. ; Skoutelis, Athanasius T. ; Papavassiliou, John T. ; Phair, John Phillip ; Bassaris, Harry P. ; Lianou, Popi E. ; Skoutelis, Athanasius T. ; Papavassiliou, John T. ; Phair, John P. / Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum. In: Journal of Infectious Diseases. 1982 ; Vol. 146, No. 1. pp. 52-55.
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abstract = "The effects of sera obtained from 18 patients with homozygous f3-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1010 ± 2.5010 (mean ± sn) compared with 17.9010 ± 8.0010 adherence in control serum (P< 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6010 ± 6.2010 (P < 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.",
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Bassaris, HP, Lianou, PE, Skoutelis, AT, Papavassiliou, JT, Phair, JP, Bassaris, HP, Lianou, PE, Skoutelis, AT, Papavassiliou, JT & Phair, JP 1982, 'Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum', Journal of Infectious Diseases, vol. 146, no. 1, pp. 52-55. https://doi.org/10.1093/infdis/146.1.52

Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum. / Bassaris, Harry P.; Lianou, Popi E.; Skoutelis, Athanasius T.; Papavassiliou, John T.; Phair, John Phillip; Bassaris, Harry P.; Lianou, Popi E.; Skoutelis, Athanasius T.; Papavassiliou, John T.; Phair, John P.

In: Journal of Infectious Diseases, Vol. 146, No. 1, 01.01.1982, p. 52-55.

Research output: Contribution to journalArticle

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AU - Skoutelis, Athanasius T.

AU - Papavassiliou, John T.

AU - Phair, John Phillip

AU - Bassaris, Harry P.

AU - Lianou, Popi E.

AU - Skoutelis, Athanasius T.

AU - Papavassiliou, John T.

AU - Phair, John P.

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N2 - The effects of sera obtained from 18 patients with homozygous f3-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1010 ± 2.5010 (mean ± sn) compared with 17.9010 ± 8.0010 adherence in control serum (P< 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6010 ± 6.2010 (P < 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.

AB - The effects of sera obtained from 18 patients with homozygous f3-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1010 ± 2.5010 (mean ± sn) compared with 17.9010 ± 8.0010 adherence in control serum (P< 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6010 ± 6.2010 (P < 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.

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