Defective adherence of polymorphonuclear leukocytes to nylon induced by thalassemic serum

Harry P. Bassaris, Popi E. Lianou, Athanasius T. Skoutelis, John T. Papavassiliou, John P. Phair, Harry P. Bassaris, Popi E. Lianou, Athanasius T. Skoutelis, John T. Papavassiliou, John P. Phair

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The effects of sera obtained from 18 patients with homozygous f3-thalassemia on the adherence and chemotaxis of normal polymorphonuclear leukocytes (PMNLs) was studied. The adherence of PMNLs suspended in thalassemic serum was 3.1010 ± 2.5010 (mean ± sn) compared with 17.9010 ± 8.0010 adherence in control serum (P< 0.00001). Zymosan-treated thalassemic serum did not generate chemotactic factors, and zymosan-treated normal serum did not induce chemotaxis of PMNLs suspended in thalassemic serum. When added to zymosan-treated normal plasma, thalassemic serum inhibited augmentation of PMNL adherence to nylon columns. These abnormalities were due to a heat-labile factor(s) in the sera of patients with thalassemia. Suspending PMNLs in heated thalassemic serum (56 C for 30 min) resulted in an increase of adherence to 14.6010 ± 6.2010 (P < 0.0001). PMNLs suspended in heated thalassemic serum responded normally to chemotactic attractants.

Original languageEnglish (US)
Pages (from-to)52-55
Number of pages4
JournalJournal of Infectious Diseases
Volume146
Issue number1
DOIs
StatePublished - Jul 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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