Degradation of complex arabinoxylans by human colonic Bacteroidetes

Gabriel V. Pereira; Ahmed M. Abdel-Hamid; Soumajit Dutta; Corina N. D’Alessandro-Gabazza; Daniel Wefers; Jacob A. Farris; Shiv Bajaj; Zdzislaw Wawrzak; Haruyuki Atomi; Roderick I. Mackie; Esteban C. Gabazza; Diwakar Shukla; Nicole M. Koropatkin; Isaac Cann

doi:10.1038/s41467-020-20737-5

Degradation of complex arabinoxylans by human colonic Bacteroidetes

Gabriel V. Pereira, Ahmed M. Abdel-Hamid, Soumajit Dutta, Corina N. D’Alessandro-Gabazza, Daniel Wefers, Jacob A. Farris, Shiv Bajaj, Zdzislaw Wawrzak, Haruyuki Atomi, Roderick I. Mackie, Esteban C. Gabazza, Diwakar Shukla, Nicole M. Koropatkin, Isaac Cann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.

Original language	English (US)
Article number	459
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-20737-5
State	Published - Dec 1 2021

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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Pereira, G. V., Abdel-Hamid, A. M., Dutta, S., D’Alessandro-Gabazza, C. N., Wefers, D., Farris, J. A., Bajaj, S., Wawrzak, Z., Atomi, H., Mackie, R. I., Gabazza, E. C., Shukla, D., Koropatkin, N. M., & Cann, I. (2021). Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nature communications, 12(1), Article 459. https://doi.org/10.1038/s41467-020-20737-5

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abstract = "Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.",

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note = "Funding Information: The research was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number RO1GM140306 to R.I.M., N.M.K., and I.C., and in part by funding from the Microbiome Metabolic Engineering Theme (MME) of the Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana Champaign. The funders had no role in the study design, data analyses, decision to publish, or preparation of the manuscript. I.C. would like to acknowledge support during sabbatical by the Top Global University Program, Kyoto University, Japan. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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Degradation of complex arabinoxylans by human colonic Bacteroidetes

Abstract

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