Degranulation of human mast cells induces an endothelial antigen central to leukocyte adhesion

L. M. Klein, R. M. Lavker, W. L. Matis, G. F. Murphy

Research output: Contribution to journalArticlepeer-review

205 Scopus citations

Abstract

To understand better the role of mast cell secretory products in the genesis of inflammation, a system was developed for in vitro degranulation of human mast cells in skin organ cultures. Within 2 hr after morphine sulfate-induced degranulation, endothelial cells lining microvessels adjacent to affected mast cells expressed an activation antigen important for endothelial-leukocyte adhesion. Identical results were obtained when other mast cell secretagogues (anti-IgE, compound 48/80, and calcium ionophore A23187) were used. Induction of this antigen was abrogated by preincubation with cromolyn sodium, an inhibitor of mast cell secretion, and by antiserum to tumor necrosis factor α. These findings indicate that degranulation of mast cells activates dermal endothelium through tumor necrosis factor-dependent mechanisms. This event may be critical to the elicitation phase of cutaneous inflammation.

Original languageEnglish (US)
Pages (from-to)8972-8976
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number22
DOIs
StatePublished - 1989

Keywords

  • cytokines
  • inflammation
  • organ culture
  • skin
  • tumor necrosis factor

ASJC Scopus subject areas

  • General

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