Dehydroepiandrosterone- induced peroxisome proliferation in the rat liver

M. Sambasiva Rao*, Sanjoy Musunuri, Janardan K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

We have investigated the effect of dehydroepiandrosterone (DHEA), a naturally occurring steroid hormone, on hepatic peroxisomes in the rat. Dietary administration of DHEA at a concentration of 0.45% for 2 weeks resulted in a 200% increase in the liver weight and a more than 5.0-fold increase in the volume density of peroxisomes. SDS-polyacrylamide gel electrophoresis of the postnuclear fraction of livers showed a marked increase in the 80,000- molecular weight polypeptide. Northern blot analysis of total RNA showed 2- and 12-fold increases, respectively, in the levels of catalase and peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme (PBE) mRNA. Administration of a single large dose of DHEA led to a 10- to 40-fold increase in PBE mRNA levels by 24 h. These changes arc qualitatively similar to those caused by a variety of structurally different peroxisome proliferators.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalPathobiology
Volume60
Issue number2
DOIs
StatePublished - Jan 1 1992

Keywords

  • Catalase morphometry
  • Hepatocytes
  • Peroxisomes
  • Steroids
  • β-Oxidation enzymes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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