TY - JOUR
T1 - Deletion of DNA encoding the first five transmembrane domains of Epstein- Barr virus latent membrane proteins 2A and 2B
AU - Longnecker, Richard
AU - Miller, Cheryl L.
AU - Tomkinson, Blake
AU - Miao, X. Qian
AU - Kieff, Elliott
PY - 1993
Y1 - 1993
N2 - A recombinant Epstein-Barr virus (EBV) was constructed, with a positive- selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.
AB - A recombinant Epstein-Barr virus (EBV) was constructed, with a positive- selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.
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U2 - 10.1128/jvi.67.8.5068-5074.1993
DO - 10.1128/jvi.67.8.5068-5074.1993
M3 - Comment/debate
C2 - 8392630
AN - SCOPUS:0027236266
SN - 0022-538X
VL - 67
SP - 5068
EP - 5074
JO - Journal of Virology
JF - Journal of Virology
IS - 8
ER -