Deletion of DNA encoding the first five transmembrane domains of Epstein- Barr virus latent membrane proteins 2A and 2B

Richard Longnecker*, Cheryl L. Miller, Blake Tomkinson, X. Qian Miao, Elliott Kieff

*Corresponding author for this work

Research output: Contribution to journalComment/debate

80 Scopus citations

Abstract

A recombinant Epstein-Barr virus (EBV) was constructed, with a positive- selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.

Original languageEnglish (US)
Pages (from-to)5068-5074
Number of pages7
JournalJournal of virology
Volume67
Issue number8
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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