Delta and sigma sites of clonal NCB20 cells do not modulate calcium uptake

R. E. West*, S. B. Freedman, G. Dawson, R. J. Miller, M. L. Villereal

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Opiate alkaloids and peptides are reported to inhibit 45Ca2+ binding to synaptic plasma membranes and uptake into brain synaptosomes. We have examined the effects of a number of opiates on 45Ca2+ uptake in a clonal cell line NCB20 which expresses multiple opioid binding sites. The cells express voltage-dependent calcium channels that are blocked by verapamil and nifedipine. In contrast to brain, 45Ca2+ uptake in these cells, in normal or high potassium medium, is unaffected by opiates. This difference may be due to the particular receptor types; the δ and σ sites of these cells do not inhibit 45Ca2+ uptake.

Original languageEnglish (US)
Pages (from-to)1335-1338
Number of pages4
JournalLife Sciences
Volume31
Issue number12-13
DOIs
StatePublished - Sep 1982

Fingerprint

Opiate Alkaloids
Calcium
Brain
Synaptic Membranes
Opioid Peptides
Synaptosomes
Calcium Channels
Cell membranes
Nifedipine
Verapamil
Opioid Analgesics
Potassium
Binding Sites
Cells
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

West, R. E. ; Freedman, S. B. ; Dawson, G. ; Miller, R. J. ; Villereal, M. L. / Delta and sigma sites of clonal NCB20 cells do not modulate calcium uptake. In: Life Sciences. 1982 ; Vol. 31, No. 12-13. pp. 1335-1338.
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West, RE, Freedman, SB, Dawson, G, Miller, RJ & Villereal, ML 1982, 'Delta and sigma sites of clonal NCB20 cells do not modulate calcium uptake', Life Sciences, vol. 31, no. 12-13, pp. 1335-1338. https://doi.org/10.1016/0024-3205(82)90375-7

Delta and sigma sites of clonal NCB20 cells do not modulate calcium uptake. / West, R. E.; Freedman, S. B.; Dawson, G.; Miller, R. J.; Villereal, M. L.

In: Life Sciences, Vol. 31, No. 12-13, 09.1982, p. 1335-1338.

Research output: Contribution to journalArticle

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