Demonstration of cystine containing protein in keratohyalin granules of the epidermis

A. G. Matoltsy, R. M. Lavker, M. N. Matoltsy

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Incorporation of cystine 3H into keratohyalin granules of newborn rat epidermis was studied by autoradiography. Sulfhydryl and disulfide groups were tested both in situ and in isolated keratohyalin granules by the use of the 2,2' dihydroxy 6,6' dinaphthyl disulfide (DDD) reagent. Labeled granules appeared in significant numbers in skin specimens taken 6 and 24 hr after injection of cystine 3H, indicating that cystine containing protein had been incorporated. Granules in situ gave a negative test whereas isolated granules gave a positive test with the DDD reagent for both sulfhydryl and disulfide groups. The negative reaction obtained in situ was attributed to blocking of the histochemical reaction rather than to the absence of cystine containing protein in keratohyalin granules as proposed by previous investigators.

Original languageEnglish (US)
Pages (from-to)406-410
Number of pages5
JournalJournal of Investigative Dermatology
Volume62
Issue number4
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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