Dependence of the structure and mechanics of metaphase chromosomes on oxidized cysteines

Adrienne Eastland, Jessica Hornick, Ryo Kawamura, Dhaval Nanavati, John F. Marko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have found that reagents that reduce oxidized cysteines lead to destabilization of metaphase chromosome folding, suggesting that chemically linked cysteine residues may play a structural role in mitotic chromosome organization, in accord with classical studies by Dounce et al. (J Theor Biol 42:275–285, 1973) and Sumner (J Cell Sci 70:177–188, 1984a). Human chromosomes isolated into buffer unfold when exposed to dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP). In micromanipulation experiments which allow us to examine the mechanics of individual metaphase chromosomes, we have found that the gel-like elastic stiffness of native metaphase chromosomes is dramatically suppressed by DTT and TCEP, even before the chromosomes become appreciably unfolded. We also report protein labeling experiments on human metaphase chromosomes which allow us to tag oxidized and reduction-sensitive cysteine residues. PAGE analysis using fluorescent labels shows a small number of labeled bands. Mass spectrometry analysis of similarly labeled proteins provides a list of candidates for proteins with oxidized cysteines involved in chromosome organization, notably including components of condensin I, cohesin, the nucleosome-interacting proteins RCC1 and RCC2, as well as the RNA/DNA-binding protein NONO/p54NRB.

Original languageEnglish (US)
Pages (from-to)339-353
Number of pages15
JournalChromosome Research
Volume24
Issue number3
DOIs
StatePublished - Sep 1 2016

Keywords

  • Chromosome structure
  • Cysteine
  • Disulfide
  • Metaphase chromosome

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Dependence of the structure and mechanics of metaphase chromosomes on oxidized cysteines'. Together they form a unique fingerprint.

Cite this