TY - JOUR
T1 - Depolarization drives β-catenin into neuronal spines promoting changes in synaptic structure and function
AU - Murase, Sachiko
AU - Mosser, Eric
AU - Schuman, Erin M.
PY - 2002/7/3
Y1 - 2002/7/3
N2 - Activity-induced changes in adhesion molecules may coordinate presynaptic and postsynaptic plasticity. Here, we demonstrate that β-catenin, which mediates interactions between cadherins and the actin cytoskeleton, moves from dendritic shafts into spines upon depolarization, increasing its association with cadherins. β-catenin's redistribution was mimicked or prevented by a tyrosine kinase or phosphatase inhibitor, respectively. Point mutations of β-catenin's tyrosine 654 altered the shaft/spine distribution: Y654F-β-catenin-GFP (phosphorylation-prevented) was concentrated in spines, whereas Y654E-β-catenin-GFP (phosphorylation-mimic) accumulated in dendritic shafts. In Y654F-expressing neurons, the PSD-95 or associated synapsin-I clusters were larger than those observed in either wild-type-β-catenin or also Y654E-expressing neurons. Y654F-expressing neurons exhibited a higher minifrequency. Thus, neural activity induces β-catenin's redistribution into spines, where it interacts with cadherin to influence synaptic size and strength.
AB - Activity-induced changes in adhesion molecules may coordinate presynaptic and postsynaptic plasticity. Here, we demonstrate that β-catenin, which mediates interactions between cadherins and the actin cytoskeleton, moves from dendritic shafts into spines upon depolarization, increasing its association with cadherins. β-catenin's redistribution was mimicked or prevented by a tyrosine kinase or phosphatase inhibitor, respectively. Point mutations of β-catenin's tyrosine 654 altered the shaft/spine distribution: Y654F-β-catenin-GFP (phosphorylation-prevented) was concentrated in spines, whereas Y654E-β-catenin-GFP (phosphorylation-mimic) accumulated in dendritic shafts. In Y654F-expressing neurons, the PSD-95 or associated synapsin-I clusters were larger than those observed in either wild-type-β-catenin or also Y654E-expressing neurons. Y654F-expressing neurons exhibited a higher minifrequency. Thus, neural activity induces β-catenin's redistribution into spines, where it interacts with cadherin to influence synaptic size and strength.
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U2 - 10.1016/S0896-6273(02)00764-X
DO - 10.1016/S0896-6273(02)00764-X
M3 - Article
C2 - 12123611
AN - SCOPUS:0037014456
VL - 35
SP - 91
EP - 105
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -