Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells

Teresa M. Perney; Robert J. Dinerstein; Richard J. Miller

doi:10.1016/0304-3940(84)90545-7

Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells

Teresa M. Perney, Robert J. Dinerstein, Richard J. Miller^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca²⁺ sensitive probe, quin2. The changes in intracellular free Ca²⁺ were induced either by depolarizing cells with high K⁺ or veratridine or by the addition of ionomycin. Ca²⁺-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca²⁺ levels, indicating that Ca²⁺ entry is most likely through voltage-sensitive calcium channels.

Original language	English (US)
Pages (from-to)	165-170
Number of pages	6
Journal	Neuroscience Letters
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(84)90545-7
State	Published - Oct 12 1984

Funding

Supported by PHS Grants DA02121 and DA02575 and GM22220. T.M.P. is supported by a Pharmacology Training Grant Predoctoral Fellowship GM07839.

Keywords

NG108-15 cells
calcium channels
dihydropyridines
intracellular free calcium
microspectrofluorometry
quin2

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(84)90545-7

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title = "Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells",

abstract = "Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca2+ sensitive probe, quin2. The changes in intracellular free Ca2+ were induced either by depolarizing cells with high K+ or veratridine or by the addition of ionomycin. Ca2+-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca2+ levels, indicating that Ca2+ entry is most likely through voltage-sensitive calcium channels.",

keywords = "NG108-15 cells, calcium channels, dihydropyridines, intracellular free calcium, microspectrofluorometry, quin2",

author = "Perney, {Teresa M.} and Dinerstein, {Robert J.} and Miller, {Richard J.}",

note = "Funding Information: Supported by PHS Grants DA02121 and DA02575 and GM22220. T.M.P. is supported by a Pharmacology Training Grant Predoctoral Fellowship GM07839.",

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doi = "10.1016/0304-3940(84)90545-7",

language = "English (US)",

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T1 - Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells

AU - Perney, Teresa M.

AU - Dinerstein, Robert J.

AU - Miller, Richard J.

N1 - Funding Information: Supported by PHS Grants DA02121 and DA02575 and GM22220. T.M.P. is supported by a Pharmacology Training Grant Predoctoral Fellowship GM07839.

PY - 1984/10/12

Y1 - 1984/10/12

N2 - Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca2+ sensitive probe, quin2. The changes in intracellular free Ca2+ were induced either by depolarizing cells with high K+ or veratridine or by the addition of ionomycin. Ca2+-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca2+ levels, indicating that Ca2+ entry is most likely through voltage-sensitive calcium channels.

AB - Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca2+ sensitive probe, quin2. The changes in intracellular free Ca2+ were induced either by depolarizing cells with high K+ or veratridine or by the addition of ionomycin. Ca2+-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca2+ levels, indicating that Ca2+ entry is most likely through voltage-sensitive calcium channels.

KW - NG108-15 cells

KW - calcium channels

KW - dihydropyridines

KW - intracellular free calcium

KW - microspectrofluorometry

KW - quin2

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Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells

Abstract

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Keywords

ASJC Scopus subject areas

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