Depolarization-induced increases in intracellular free calcium detected in single cultured neuronal cells

Teresa M. Perney, Robert J. Dinerstein, Richard J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Microspectrofluorometry was used to examine intracellular free calcium changes in single NG108-15 neurons loaded with the Ca2+ sensitive probe, quin2. The changes in intracellular free Ca2+ were induced either by depolarizing cells with high K+ or veratridine or by the addition of ionomycin. Ca2+-free medium and various inorganic and organic calcium-channel blocking agents blocked changes in intracellular free Ca2+ levels, indicating that Ca2+ entry is most likely through voltage-sensitive calcium channels.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalNeuroscience Letters
Volume51
Issue number2
DOIs
StatePublished - Oct 12 1984

Keywords

  • NG108-15 cells
  • calcium channels
  • dihydropyridines
  • intracellular free calcium
  • microspectrofluorometry
  • quin2

ASJC Scopus subject areas

  • Neuroscience(all)

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