Depth-resolved measurement of mucosal microvascular blood content using low-coherence enhanced backscattering spectroscopy

Andrew J. Radosevich, Vladimir M. Turzhitsky, Nikhil N. Mutyal, Jeremy D. Rogers, Valentina Stoyneva, Ashish Kumar Tiwari, Mart De La Cruz, Dhananjay P. Kunte, Ramesh K. Wali, Hemant K. Roy, Vadim Backman

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Low-coherence enhanced backscattering (LEBS) spectroscopy is a light scattering technique which uses partial spatial coherence broadband illumination to interrogate the optical properties at sub-diffusion length scales. In this work, we present a post-processing technique which isolates the hemoglobin concentration at different depths within a sample using a single spectroscopic LEBS measurement with a fixed spatial coherence of illumination. We verify the method with scattering (spectralon reflectance standard and polystyrene microspheres) and absorbing (hemoglobin) phantoms. We then demonstrate the relevance of this method for quantifying hemoglobin content as a function of depth within biological tissue using the azoxymethane treated animal model of colorectal cancer.

Original languageEnglish (US)
Pages (from-to)1196-1208
Number of pages13
JournalBiomedical Optics Express
Volume1
Issue number4
DOIs
StatePublished - Nov 1 2010

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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