Derivation of LIF-independent mouse iPS cells with modified Oct4

Hiroyuki Hirai; Meri Firpo; Nobuaki Kikyo

doi:10.1016/j.scr.2015.08.005

Derivation of LIF-independent mouse iPS cells with modified Oct4

Hiroyuki Hirai, Meri Firpo, Nobuaki Kikyo^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

Abstract

It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120. days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.

Original language	English (US)
Pages (from-to)	384-386
Number of pages	3
Journal	Stem Cell Research
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/j.scr.2015.08.005
State	Published - Sep 1 2015

ASJC Scopus subject areas

Cell Biology
Developmental Biology

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10.1016/j.scr.2015.08.005

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title = "Derivation of LIF-independent mouse iPS cells with modified Oct4",

abstract = "It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120. days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.",

author = "Hiroyuki Hirai and Meri Firpo and Nobuaki Kikyo",

note = "Funding Information: We thank Toshio Kitamura for Plat-E cells and the pMXs-IP vector. Histology and karyotyping were performed at the Comparative Pathology Shared Resource and the Cytogenomics Shared Resource, respectively at the University of Minnesota Masonic Cancer Center with the support by the NIH grant P30 CA077598 . M.F. and N.K. were supported by Richard M. Schulze Family Foundation . N.K. was supported by the NIH ( R01 GM098294 and R21 CA187232 ); Engdahl Family Foundation ; Grain-in-Aid of Research, Artistry and Scholarship, University of Minnesota ( 22802 ); and the University of Minnesota Foundation ( 4160-9227-13 ). Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

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doi = "10.1016/j.scr.2015.08.005",

language = "English (US)",

volume = "15",

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journal = "Stem Cell Research",

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AU - Hirai, Hiroyuki

AU - Firpo, Meri

AU - Kikyo, Nobuaki

N1 - Funding Information: We thank Toshio Kitamura for Plat-E cells and the pMXs-IP vector. Histology and karyotyping were performed at the Comparative Pathology Shared Resource and the Cytogenomics Shared Resource, respectively at the University of Minnesota Masonic Cancer Center with the support by the NIH grant P30 CA077598 . M.F. and N.K. were supported by Richard M. Schulze Family Foundation . N.K. was supported by the NIH ( R01 GM098294 and R21 CA187232 ); Engdahl Family Foundation ; Grain-in-Aid of Research, Artistry and Scholarship, University of Minnesota ( 22802 ); and the University of Minnesota Foundation ( 4160-9227-13 ). Publisher Copyright: © 2015 The Authors.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120. days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.

AB - It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120. days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.

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JF - Stem Cell Research

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Derivation of LIF-independent mouse iPS cells with modified Oct4

Abstract

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