Design, Assembly, Production, and Transfection of Synthetic Modified mRNA

Sanders Oh*, John A. Kessler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Expression of exogenous genes using synthetic modified mRNAs has a superior safety profile and higher efficiency than DNA-based techniques. However, mRNA stability and immunogenicity have been significant barriers for wide spread use. The modified mRNAs in this protocol address these shortcomings and can be used to direct cell fate specification or cellular reprogramming.

Original languageEnglish (US)
Pages (from-to)29-43
Number of pages15
JournalMethods
Volume133
DOIs
StatePublished - Jan 15 2018

Keywords

  • mRNA protocol
  • mRNA reprogramming
  • mRNA synthesis
  • mRNA transfection
  • messenger RNA
  • modified mRNA
  • reprogramming factors
  • synthetic mRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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