Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples

Bryan Schmidt, Goher Mahmud, Siowling Soh, Sun Hee Kim, Taylor Page, Thomas V O'Halloran, Bartosz A. Grzybowski, Brian M Hoffman

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Rapid freeze-quench (RFQ) trapping of short-lived reaction intermediates for spectroscopic study plays an important role in the characterization of biological reactions. Recently, there has been considerable effort to achieve sub-millisecond reaction deadtimes. We present here a new, robust, high-velocity microfluidic mixer that enables such rapid freeze-quenching. It is a based on the mixing method of two impinging jets commonly used in reaction injection molding of plastics. This method achieves efficient mixing by inducing chaotic flow at relatively low Reynolds numbers (Re = 140). We present the first mathematical simulation and microscopic visualization of mixing in such RFQ micromixers, the results of which show that the impinging solutions efficiently mix within the mixing chamber. These tests, along with a practical demonstration in an RFQ setup that involves copper wheels, show that this new mixer can in practice provide reaction deadtimes as low as 100 μs.

Original languageEnglish (US)
Pages (from-to)415-425
Number of pages11
JournalApplied Magnetic Resonance
Volume40
Issue number4
DOIs
StatePublished - Aug 1 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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