Design Rules for Template-Confined DNA-Mediated Nanoparticle Assembly

Wenjie Zhou, Qing Yuan Lin, Jarad A. Mason, Vinayak P. Dravid, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Template-based strategies are becoming increasingly important for controlling the position of nanoparticle-based (NP-based) structures on surfaces for a wide variety of encoding and device fabrication strategies. Thus, there is an increasing need to understand the behavior of NPs in confined spaces. Herein, a systematic investigation of the diffusion and adsorption properties of DNA-modified NPs is presented in lithographically defined, high-aspect-ratio pores using a template-confined, DNA-mediated assembly. Leveraging the sequence-specific binding affinity of DNA, it is discovered that although NP adsorption in deep polymer pores follows a traditional Langmuir adsorption model when under thermodynamic control, such NPs kinetically follow Fick's classical law of diffusion. Importantly, these observations allow one to establish design rules for template-confined, DNA-mediated NP assembly on substrates based on pore dimensions, NP size and shape, NP concentration, temperature, and time. As a proof-of-concept example, these design rules are used to engineer a vertical, four-layer assembly consisting of individual octahedral NPs stacked on top of one another, with in-plane positioning defined by pores generated by e-beam lithography.

Original languageEnglish (US)
Article number1802742
Issue number44
StatePublished - Nov 2 2018


  • DNA-mediated assembly
  • adsorption
  • gold nanoparticles
  • kinetics
  • thermodynamics

ASJC Scopus subject areas

  • General Chemistry
  • Engineering (miscellaneous)
  • Biotechnology
  • General Materials Science
  • Biomaterials


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