Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations

James F. Matthaei; Frank DiMaio; Jeffrey J. Richards; Lilo D. Pozzo; David Baker; François Baneyx

doi:10.1021/acs.nanolett.5b01499

Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations

James F. Matthaei, Frank DiMaio, Jeffrey J. Richards, Lilo D. Pozzo, David Baker, François Baneyx^*

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

We have combined fusion of oligomers with cyclic symmetry and alanine substitutions to eliminate clashes and produce proteins that self-assemble into 2-D arrays upon addition of calcium ions. Using TEM, AFM, small-angle X-ray scattering, and fluorescence microscopy, we show that the designed lattices which are 5 nm high with p3 space group symmetry and 7.25 nm periodicity self-assemble into structures that can exceed 100 ∼m in characteristic length. The versatile strategy, experimental approach, and hexagonal arrays described herein should prove valuable for the engineering of functional nanostructured materials in 2-D.

Original language	English (US)
Pages (from-to)	5235-5239
Number of pages	5
Journal	Nano letters
Volume	15
Issue number	8
DOIs	https://doi.org/10.1021/acs.nanolett.5b01499
State	Published - Aug 12 2015

Keywords

2-D materials
Protein design
artificial S-layers
bionanotechnology
self-assembly

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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AU - DiMaio, Frank

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AU - Baker, David

AU - Baneyx, François

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KW - artificial S-layers

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