Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations

James F. Matthaei, Frank DiMaio, Jeffrey J. Richards, Lilo D. Pozzo, David Baker, François Baneyx*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We have combined fusion of oligomers with cyclic symmetry and alanine substitutions to eliminate clashes and produce proteins that self-assemble into 2-D arrays upon addition of calcium ions. Using TEM, AFM, small-angle X-ray scattering, and fluorescence microscopy, we show that the designed lattices which are 5 nm high with p3 space group symmetry and 7.25 nm periodicity self-assemble into structures that can exceed 100 ∼m in characteristic length. The versatile strategy, experimental approach, and hexagonal arrays described herein should prove valuable for the engineering of functional nanostructured materials in 2-D.

Original languageEnglish (US)
Pages (from-to)5235-5239
Number of pages5
JournalNano letters
Volume15
Issue number8
DOIs
StatePublished - Aug 12 2015

Keywords

  • 2-D materials
  • Protein design
  • artificial S-layers
  • bionanotechnology
  • self-assembly

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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