Detecting apoptosis in Drosophila tissues and cells

Tatevik Sarkissian; Allison Timmons; Richa Arya; Eltyeb Abdelwahid; Kristin White

doi:10.1016/j.ymeth.2014.02.033

Detecting apoptosis in Drosophila tissues and cells

Tatevik Sarkissian, Allison Timmons, Richa Arya, Eltyeb Abdelwahid, Kristin White^*

^*Corresponding author for this work

Feinberg Cardiovascular Research Institute

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.

Original language	English (US)
Pages (from-to)	89-96
Number of pages	8
Journal	Methods
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2014.02.033
State	Published - Jun 15 2014

Keywords

Apoptosis
Caspase
Drosophila
TUNEL

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Molecular Biology

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10.1016/j.ymeth.2014.02.033

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abstract = "In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.",

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AU - Sarkissian, Tatevik

AU - Timmons, Allison

AU - Arya, Richa

AU - Abdelwahid, Eltyeb

AU - White, Kristin

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AB - In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.

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KW - Caspase

KW - Drosophila

KW - TUNEL

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Detecting apoptosis in Drosophila tissues and cells

Abstract

Keywords

ASJC Scopus subject areas

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