Detecting apoptosis in Drosophila tissues and cells

Tatevik Sarkissian, Allison Timmons, Richa Arya, Eltyeb Abdelwahid, Kristin White*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalMethods
Volume68
Issue number1
DOIs
StatePublished - Jun 15 2014

Keywords

  • Apoptosis
  • Caspase
  • Drosophila
  • TUNEL

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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