Detection and characterization of a multicopper oxidase from nitrosomonas Europaea

Thomas J. Lawton, Amy C Rosenzweig

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

Original languageEnglish (US)
Pages (from-to)423-433
Number of pages11
JournalMethods in Enzymology
Volume496
DOIs
StatePublished - May 5 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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