Abstract
Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.
Original language | English (US) |
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Title of host publication | Methods in Enzymology |
Publisher | Academic Press Inc |
Pages | 423-433 |
Number of pages | 11 |
DOIs | |
State | Published - 2011 |
Publication series
Name | Methods in Enzymology |
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Volume | 496 |
ISSN (Print) | 0076-6879 |
Funding
We thank Daniel Arp and Luis Sayavedra-Soto for support throughout this project. This work was supported by the Agriculture and Food Research Initiative competitive Grant No. 2010-65115-20380 from the USDA National Institute of Food and Agriculture.
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology