Detection and characterization of a multicopper oxidase from nitrosomonas Europaea

Thomas J. Lawton; Amy C. Rosenzweig

doi:10.1016/B978-0-12-386489-5.00017-8

Detection and characterization of a multicopper oxidase from nitrosomonas Europaea

Thomas J. Lawton, Amy C. Rosenzweig

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Scopus citations

Abstract

Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

Original language	English (US)
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc
Pages	423-433
Number of pages	11
DOIs	https://doi.org/10.1016/B978-0-12-386489-5.00017-8
State	Published - 2011

Publication series

Name	Methods in Enzymology
Volume	496
ISSN (Print)	0076-6879

Funding

We thank Daniel Arp and Luis Sayavedra-Soto for support throughout this project. This work was supported by the Agriculture and Food Research Initiative competitive Grant No. 2010-65115-20380 from the USDA National Institute of Food and Agriculture.

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/B978-0-12-386489-5.00017-8

Cite this

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title = "Detection and characterization of a multicopper oxidase from nitrosomonas Europaea",

abstract = "Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.",

author = "Lawton, {Thomas J.} and Rosenzweig, {Amy C.}",

note = "Funding Information: We thank Daniel Arp and Luis Sayavedra-Soto for support throughout this project. This work was supported by the Agriculture and Food Research Initiative competitive Grant No. 2010-65115-20380 from the USDA National Institute of Food and Agriculture. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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AU - Lawton, Thomas J.

AU - Rosenzweig, Amy C.

N1 - Funding Information: We thank Daniel Arp and Luis Sayavedra-Soto for support throughout this project. This work was supported by the Agriculture and Food Research Initiative competitive Grant No. 2010-65115-20380 from the USDA National Institute of Food and Agriculture. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2011

Y1 - 2011

N2 - Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

AB - Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

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ER -

Detection and characterization of a multicopper oxidase from nitrosomonas Europaea

Abstract

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