Detection and characterization of a multicopper oxidase from nitrosomonas Europaea

Thomas J. Lawton, Amy C. Rosenzweig

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Pages423-433
Number of pages11
DOIs
StatePublished - 2011

Publication series

NameMethods in Enzymology
Volume496
ISSN (Print)0076-6879

Funding

We thank Daniel Arp and Luis Sayavedra-Soto for support throughout this project. This work was supported by the Agriculture and Food Research Initiative competitive Grant No. 2010-65115-20380 from the USDA National Institute of Food and Agriculture.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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