Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.
|Original language||English (US)|
|Number of pages||11|
|Journal||Methods in Enzymology|
|State||Published - May 5 2011|
ASJC Scopus subject areas
- Molecular Biology