TY - JOUR
T1 - Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies
T2 - Potential markers of embryo developmental competence
AU - Jiao, Ze Xu
AU - Woodruff, Teresa K.
N1 - Funding Information:
This work was supported by grants ( RL1HD058295 and U54HD041857 ) from the National Institutes of Health through the Eunice Kennedy Shriver National Institute of Child Health and Human Development .
PY - 2013/6
Y1 - 2013/6
N2 - Objective: To test the hypothesis that quantification of messenger RNAs originating from the second polar body (PB2) provides a noninvasive tool for assessing embryo quality. Design: Prospective study. Setting: Hospital-based academic research laboratory. Animal(s): CD1 female mice. Intervention(s): Metaphase II oocytes obtained from 7- to 8-week-old mice after pregnant mare's serum gonadotropin and hCG priming. After in vitro fertilization, the PB2 was biopsied from zygote, followed by reverse transcription. Real-time polymerase chain reaction was performed to quantify gene expression levels in single PB2. The sibling zygotes were continuously cultured to blastocyst stage. Main Outcome Measure(s): Embryo developmental competence and six maternal-effect gene (Dnmt1, Mater, Nobox, Npm2, Tcl1, and Zar1) transcripts in the PB2. Result(s): Second polar body messenger RNA was detected in all candidate genes. Transcripts that were present in greater abundance in the zygote were more likely to be detected in quantitative polymerase chain reaction replicates from single PB2. Four candidate genes (Dnmt1, Nobox, Npm2, and Tcl1) expression levels in PB 2 between two groups (two-cell embryo vs. blastocyts) approached statistical significance. Conclusion(s): Second polar bodies may contain a representative transcript profile to that of the zygote after fertilization. Differences in gene expression in PB2 may be potential biomarkers of embryo quality.
AB - Objective: To test the hypothesis that quantification of messenger RNAs originating from the second polar body (PB2) provides a noninvasive tool for assessing embryo quality. Design: Prospective study. Setting: Hospital-based academic research laboratory. Animal(s): CD1 female mice. Intervention(s): Metaphase II oocytes obtained from 7- to 8-week-old mice after pregnant mare's serum gonadotropin and hCG priming. After in vitro fertilization, the PB2 was biopsied from zygote, followed by reverse transcription. Real-time polymerase chain reaction was performed to quantify gene expression levels in single PB2. The sibling zygotes were continuously cultured to blastocyst stage. Main Outcome Measure(s): Embryo developmental competence and six maternal-effect gene (Dnmt1, Mater, Nobox, Npm2, Tcl1, and Zar1) transcripts in the PB2. Result(s): Second polar body messenger RNA was detected in all candidate genes. Transcripts that were present in greater abundance in the zygote were more likely to be detected in quantitative polymerase chain reaction replicates from single PB2. Four candidate genes (Dnmt1, Nobox, Npm2, and Tcl1) expression levels in PB 2 between two groups (two-cell embryo vs. blastocyts) approached statistical significance. Conclusion(s): Second polar bodies may contain a representative transcript profile to that of the zygote after fertilization. Differences in gene expression in PB2 may be potential biomarkers of embryo quality.
KW - Infertility
KW - embryo quality
KW - maternal-effect genes
KW - meiosis
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U2 - 10.1016/j.fertnstert.2013.02.003
DO - 10.1016/j.fertnstert.2013.02.003
M3 - Article
C2 - 23465709
AN - SCOPUS:84878475967
SN - 0015-0282
VL - 99
SP - 2055
EP - 2061
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 7
ER -