Detection and tracking of dual-labeled hiv particles using wide-field live cell imaging to follow viral core integrity

João I. Mamede, Thomas J. Hope

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages49-59
Number of pages11
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)1064-3745

Keywords

  • HIV early steps of infection
  • HIV fusion
  • HIV uncoating
  • HIV viral core
  • Live cell imaging
  • Wide-field microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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