Detection of anti-chlamydia pneumoniae ige in children with reactive airway disease

Cara C. Wilson; Johnson T. Wong; Daren D. Girard; Debra P. Merrill; Mark Dynan; Donna D. An; Spyros A. Kalams; R. Paul Johnson; Martin S. Hirsch; Richard T. D’ Aquila; Bruce D. Walker

doi:10.1093/infdis/172.1.88

Detection of anti-chlamydia pneumoniae ige in children with reactive airway disease

Cara C. Wilson^*, Johnson T. Wong, Daren D. Girard, Debra P. Merrill, Mark Dynan, Donna D. An, Spyros A. Kalams, R. Paul Johnson, Martin S. Hirsch, Richard T. D’ Aquila, Bruce D. Walker

^*Corresponding author for this work

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Expansion of CD4 lymphocytes from human immunodeficiency virus type 1 (HIV-1)-infected persons ex vivo has been limited by enhanced virus replication and cell death. The successful expansion of functional CD4 lymphocytes from HIV-1-infected persons has now been accomplished using a bispecific monoclonal antibody to CD3 and CD8 in combination with three antiretroviral agents. CD4 lymphocytes were polyclonally expanded by a factor of 10³-10⁷ during 4-8 weeks in culture. Supernatants from most cultures were persistently HIV-1 p24 antigen-negative by day 14 and remained negative despite removal of antiretroviral agents at day 28. In such cultures, HIV-1 could not be recovered by cocultivation, and amounts of HIV-1 DNA declined or remained stable at low levels, eventually becoming undetectable in 2 cases. This approach establishes the feasibility of CD4 lymphocyte expansion in persons with HIV disease and may be useful for immune-based or gene therapies.

Original language	English (US)
Pages (from-to)	88-96
Number of pages	9
Journal	Journal of Infectious Diseases
Volume	172
Issue number	1
DOIs	https://doi.org/10.1093/infdis/172.1.88
State	Published - Jul 1995

Funding

Received 26 September 1994; revised 2 February 1995. Presented in part: First National Conference on Human Retroviruses and Related Infections, Washington, DC, December 1993. Written informed consent was obtained from all subjects studied following the guidelines established by the Massachusetts General Hospital Human Studies Committee. Grantsupport: NationalInstitutesofHealth(AI-36550,AI-366ll,AI-30914, CA-12464), Pediatric AIDS Foundation Ariel Project, and NIAID AIDS Institutional Training Grant AI-07061. Reprints or correspondence: Dr. Cara C. Wilson, Infectious Disease Unit, Gray 5, Massachusetts General Hospital, Boston, MA 02114. * Present affiliation: New England Regional Primate Research Center, Southborough, and Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts.

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/172.1.88

Cite this

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title = "Detection of anti-chlamydia pneumoniae ige in children with reactive airway disease",

abstract = "Expansion of CD4 lymphocytes from human immunodeficiency virus type 1 (HIV-1)-infected persons ex vivo has been limited by enhanced virus replication and cell death. The successful expansion of functional CD4 lymphocytes from HIV-1-infected persons has now been accomplished using a bispecific monoclonal antibody to CD3 and CD8 in combination with three antiretroviral agents. CD4 lymphocytes were polyclonally expanded by a factor of 103-107 during 4-8 weeks in culture. Supernatants from most cultures were persistently HIV-1 p24 antigen-negative by day 14 and remained negative despite removal of antiretroviral agents at day 28. In such cultures, HIV-1 could not be recovered by cocultivation, and amounts of HIV-1 DNA declined or remained stable at low levels, eventually becoming undetectable in 2 cases. This approach establishes the feasibility of CD4 lymphocyte expansion in persons with HIV disease and may be useful for immune-based or gene therapies.",

author = "Wilson, {Cara C.} and Wong, {Johnson T.} and Girard, {Daren D.} and Merrill, {Debra P.} and Mark Dynan and An, {Donna D.} and Kalams, {Spyros A.} and Johnson, {R. Paul} and Hirsch, {Martin S.} and {D{\textquoteright} Aquila}, {Richard T.} and Walker, {Bruce D.}",

note = "Funding Information: Received 26 September 1994; revised 2 February 1995. Presented in part: First National Conference on Human Retroviruses and Related Infections, Washington, DC, December 1993. Written informed consent was obtained from all subjects studied following the guidelines established by the Massachusetts General Hospital Human Studies Committee. Grantsupport: NationalInstitutesofHealth(AI-36550,AI-366ll,AI-30914, CA-12464), Pediatric AIDS Foundation Ariel Project, and NIAID AIDS Institutional Training Grant AI-07061. Reprints or correspondence: Dr. Cara C. Wilson, Infectious Disease Unit, Gray 5, Massachusetts General Hospital, Boston, MA 02114. * Present affiliation: New England Regional Primate Research Center, Southborough, and Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts.",

year = "1995",

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doi = "10.1093/infdis/172.1.88",

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AU - Wilson, Cara C.

AU - Wong, Johnson T.

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AU - Dynan, Mark

AU - An, Donna D.

AU - Kalams, Spyros A.

AU - Johnson, R. Paul

AU - Hirsch, Martin S.

AU - D’ Aquila, Richard T.

AU - Walker, Bruce D.

N1 - Funding Information: Received 26 September 1994; revised 2 February 1995. Presented in part: First National Conference on Human Retroviruses and Related Infections, Washington, DC, December 1993. Written informed consent was obtained from all subjects studied following the guidelines established by the Massachusetts General Hospital Human Studies Committee. Grantsupport: NationalInstitutesofHealth(AI-36550,AI-366ll,AI-30914, CA-12464), Pediatric AIDS Foundation Ariel Project, and NIAID AIDS Institutional Training Grant AI-07061. Reprints or correspondence: Dr. Cara C. Wilson, Infectious Disease Unit, Gray 5, Massachusetts General Hospital, Boston, MA 02114. * Present affiliation: New England Regional Primate Research Center, Southborough, and Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts.

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N2 - Expansion of CD4 lymphocytes from human immunodeficiency virus type 1 (HIV-1)-infected persons ex vivo has been limited by enhanced virus replication and cell death. The successful expansion of functional CD4 lymphocytes from HIV-1-infected persons has now been accomplished using a bispecific monoclonal antibody to CD3 and CD8 in combination with three antiretroviral agents. CD4 lymphocytes were polyclonally expanded by a factor of 103-107 during 4-8 weeks in culture. Supernatants from most cultures were persistently HIV-1 p24 antigen-negative by day 14 and remained negative despite removal of antiretroviral agents at day 28. In such cultures, HIV-1 could not be recovered by cocultivation, and amounts of HIV-1 DNA declined or remained stable at low levels, eventually becoming undetectable in 2 cases. This approach establishes the feasibility of CD4 lymphocyte expansion in persons with HIV disease and may be useful for immune-based or gene therapies.

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Detection of anti-chlamydia pneumoniae ige in children with reactive airway disease

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