Detection of anti-chlamydia pneumoniae ige in children with reactive airway disease

Cara C. Wilson*, Johnson T. Wong, Daren D. Girard, Debra P. Merrill, Mark Dynan, Donna D. An, Spyros A. Kalams, R. Paul Johnson, Martin S. Hirsch, Richard T. D’ Aquila, Bruce D. Walker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Expansion of CD4 lymphocytes from human immunodeficiency virus type 1 (HIV-1)-infected persons ex vivo has been limited by enhanced virus replication and cell death. The successful expansion of functional CD4 lymphocytes from HIV-1-infected persons has now been accomplished using a bispecific monoclonal antibody to CD3 and CD8 in combination with three antiretroviral agents. CD4 lymphocytes were polyclonally expanded by a factor of 103-107 during 4-8 weeks in culture. Supernatants from most cultures were persistently HIV-1 p24 antigen-negative by day 14 and remained negative despite removal of antiretroviral agents at day 28. In such cultures, HIV-1 could not be recovered by cocultivation, and amounts of HIV-1 DNA declined or remained stable at low levels, eventually becoming undetectable in 2 cases. This approach establishes the feasibility of CD4 lymphocyte expansion in persons with HIV disease and may be useful for immune-based or gene therapies.

Original languageEnglish (US)
Pages (from-to)88-96
Number of pages9
JournalJournal of Infectious Diseases
Volume172
Issue number1
DOIs
StatePublished - Jul 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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