Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence

Joe Mazzulli*, Lena Burbulla, Dimitri Krainc, Harry Ischiropoulos

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aging and oxidative stress are two prominent pathological mechanisms for Parkinson's disease (PD) that are strongly associated with the degeneration of dopamine (DA) neurons in the midbrain. DA and other catechols readily oxidize into highly reactive o-quinone species that are precursors of neuromelanin (NM) pigment and under pathological conditions can modify and damage macromolecules. The role of DA oxidation in PD pathogenesis remains unclear in part due to the lack of appropriate disease models and the absence of a simple method for the quantification of DA-derived oxidants. Here, we describe a rapid, simple, and reproducible method for the quantification of o-quinones in cells and tissues that relies on the near-infrared fluorescent properties of these species. Importantly, we demonstrate that catechol-derived oxidants can be quantified in human neuroblastoma cells and midbrain dopamine neurons derived from induced pluripotent stem cells, providing a novel model to study the downstream actions of o-quinones. This method should facilitate further study of oxidative stress and DA oxidation in PD and related diseases that affect the dopaminergic system.

Original languageEnglish (US)
Pages (from-to)2399-2405
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number4
DOIs
StatePublished - Feb 16 2016

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Quinones
Dopamine
Fluorescence
Infrared radiation
Proteins
Oxidative stress
Oxidants
Neurons
Catechols
Oxidation
Stem cells
Macromolecules
Pigments
Aging of materials
Tissue

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence",
abstract = "Aging and oxidative stress are two prominent pathological mechanisms for Parkinson's disease (PD) that are strongly associated with the degeneration of dopamine (DA) neurons in the midbrain. DA and other catechols readily oxidize into highly reactive o-quinone species that are precursors of neuromelanin (NM) pigment and under pathological conditions can modify and damage macromolecules. The role of DA oxidation in PD pathogenesis remains unclear in part due to the lack of appropriate disease models and the absence of a simple method for the quantification of DA-derived oxidants. Here, we describe a rapid, simple, and reproducible method for the quantification of o-quinones in cells and tissues that relies on the near-infrared fluorescent properties of these species. Importantly, we demonstrate that catechol-derived oxidants can be quantified in human neuroblastoma cells and midbrain dopamine neurons derived from induced pluripotent stem cells, providing a novel model to study the downstream actions of o-quinones. This method should facilitate further study of oxidative stress and DA oxidation in PD and related diseases that affect the dopaminergic system.",
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Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence. / Mazzulli, Joe; Burbulla, Lena; Krainc, Dimitri; Ischiropoulos, Harry.

In: Analytical Chemistry, Vol. 88, No. 4, 16.02.2016, p. 2399-2405.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence

AU - Mazzulli, Joe

AU - Burbulla, Lena

AU - Krainc, Dimitri

AU - Ischiropoulos, Harry

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AB - Aging and oxidative stress are two prominent pathological mechanisms for Parkinson's disease (PD) that are strongly associated with the degeneration of dopamine (DA) neurons in the midbrain. DA and other catechols readily oxidize into highly reactive o-quinone species that are precursors of neuromelanin (NM) pigment and under pathological conditions can modify and damage macromolecules. The role of DA oxidation in PD pathogenesis remains unclear in part due to the lack of appropriate disease models and the absence of a simple method for the quantification of DA-derived oxidants. Here, we describe a rapid, simple, and reproducible method for the quantification of o-quinones in cells and tissues that relies on the near-infrared fluorescent properties of these species. Importantly, we demonstrate that catechol-derived oxidants can be quantified in human neuroblastoma cells and midbrain dopamine neurons derived from induced pluripotent stem cells, providing a novel model to study the downstream actions of o-quinones. This method should facilitate further study of oxidative stress and DA oxidation in PD and related diseases that affect the dopaminergic system.

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