TY - JOUR
T1 - Detection of HIV-1 DNA in pediatric AIDS brain tissue by two-step ISPCR
AU - Sharer, Leroy R.
AU - Saito, Yoshihiro
AU - Epstein, Leon G.
AU - Blumberg, Benjamin M.
PY - 1994
Y1 - 1994
N2 - In order to detect latent infection in neurons or other cell types in formalin-fixed brain tissue, we performed polymerase chain reaction amplification with incorporation of digoxigenin-conjugated deoxynucleotides, followed by in situ hybridization with biotinylated probes. The use of this two-step technique in brain tissue from a child with severe HIV-1 encephalitis revealed signal in both nuclear and perinuclear regions of cells identified as monocytes and astrocytes, and also in perineuronal satellite cells of glial morphology, but HIV-1 infection of neurons was not detected.
AB - In order to detect latent infection in neurons or other cell types in formalin-fixed brain tissue, we performed polymerase chain reaction amplification with incorporation of digoxigenin-conjugated deoxynucleotides, followed by in situ hybridization with biotinylated probes. The use of this two-step technique in brain tissue from a child with severe HIV-1 encephalitis revealed signal in both nuclear and perinuclear regions of cells identified as monocytes and astrocytes, and also in perineuronal satellite cells of glial morphology, but HIV-1 infection of neurons was not detected.
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U2 - 10.1016/S0960-5428(06)80268-8
DO - 10.1016/S0960-5428(06)80268-8
M3 - Article
C2 - 7874396
AN - SCOPUS:0028073846
SN - 0960-5428
VL - 4
SP - 283
EP - 285
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
IS - 3
ER -