Detection of HIV-1 DNA in pediatric AIDS brain tissue by two-step ISPCR

Leroy R. Sharer*, Yoshihiro Saito, Leon G. Epstein, Benjamin M. Blumberg

*Corresponding author for this work

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

In order to detect latent infection in neurons or other cell types in formalin-fixed brain tissue, we performed polymerase chain reaction amplification with incorporation of digoxigenin-conjugated deoxynucleotides, followed by in situ hybridization with biotinylated probes. The use of this two-step technique in brain tissue from a child with severe HIV-1 encephalitis revealed signal in both nuclear and perinuclear regions of cells identified as monocytes and astrocytes, and also in perineuronal satellite cells of glial morphology, but HIV-1 infection of neurons was not detected.

Original languageEnglish (US)
Pages (from-to)283-285
Number of pages3
JournalAdvances in Neuroimmunology
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology
  • Neuroscience(all)

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