Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay

C. R. Zeiss; R. C. Radin; J. E. Williams; D. Levitz; J. P. Phair

doi:10.1128/jcm.15.1.93-96.1982

Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay

C. R. Zeiss, R. C. Radin, J. E. Williams, D. Levitz, J. P. Phair

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Sera from patients with active tuberculosis and sera from appropriate control individuals were assayed for immunoglobulin G antibody activity to purified protein derivative by a polystyrene tube radioimmunoassay and an enzyme-linked immunosorbent assay. Both assays showed a marked increase in immunoglobulin G antibody activity in patients with active tuberculosis. There was not overlap between the values for the patient group and the values for the purified protein derivative skin test-positive control individuals. The replication of these assays was excellent, and both could provide quantitative measurements of immunoglobulin G antibody activity to purified protein derivative antigen within 24 h. These techniques have potential as rapid diagnostic aids in evaluating patients with suspected active tuberculosis.

Original language	English (US)
Pages (from-to)	93-96
Number of pages	4
Journal	Unknown Journal
Volume	15
Issue number	1
DOIs	https://doi.org/10.1128/jcm.15.1.93-96.1982
State	Published - 1982

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.15.1.93-96.1982

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AU - Levitz, D.

AU - Phair, J. P.

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AB - Sera from patients with active tuberculosis and sera from appropriate control individuals were assayed for immunoglobulin G antibody activity to purified protein derivative by a polystyrene tube radioimmunoassay and an enzyme-linked immunosorbent assay. Both assays showed a marked increase in immunoglobulin G antibody activity in patients with active tuberculosis. There was not overlap between the values for the patient group and the values for the purified protein derivative skin test-positive control individuals. The replication of these assays was excellent, and both could provide quantitative measurements of immunoglobulin G antibody activity to purified protein derivative antigen within 24 h. These techniques have potential as rapid diagnostic aids in evaluating patients with suspected active tuberculosis.

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Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay

Abstract

ASJC Scopus subject areas

UN SDGs

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