Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

Igor J. Koralnik*, Jörn E. Schmitz, Michelle A. Lifton, Meryl A. Forman, Norman L. Letvin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalJournal of neurovirology
Volume5
Issue number4
DOIs
StatePublished - Aug 1999

Keywords

  • Cell sorting
  • HIV
  • JC virus
  • Peripheral blood mononuclear cells
  • Polymerase chain reaction
  • Progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

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