Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

Igor J. Koralnik; Jörn E. Schmitz; Michelle A. Lifton; Meryl A. Forman; Norman L. Letvin

doi:10.3109/13550289909029484

Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

Igor J. Koralnik^*, Jörn E. Schmitz, Michelle A. Lifton, Meryl A. Forman, Norman L. Letvin

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.

Original language	English (US)
Pages (from-to)	430-435
Number of pages	6
Journal	Journal of neurovirology
Volume	5
Issue number	4
DOIs	https://doi.org/10.3109/13550289909029484
State	Published - Aug 1999

Funding

Keywords

Cell sorting
HIV
JC virus
Peripheral blood mononuclear cells
Polymerase chain reaction
Progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

Clinical Neurology
Neurology
Cellular and Molecular Neuroscience
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/13550289909029484

Cite this

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title = "Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals",

abstract = "While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.",

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author = "Koralnik, {Igor J.} and Schmitz, {J{\"o}rn E.} and Lifton, {Michelle A.} and Forman, {Meryl A.} and Letvin, {Norman L.}",

year = "1999",

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T1 - Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

AU - Koralnik, Igor J.

AU - Schmitz, Jörn E.

AU - Lifton, Michelle A.

AU - Forman, Meryl A.

AU - Letvin, Norman L.

PY - 1999/8

Y1 - 1999/8

N2 - While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.

AB - While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.

KW - Cell sorting

KW - HIV

KW - JC virus

KW - Peripheral blood mononuclear cells

KW - Polymerase chain reaction

KW - Progressive multifocal leukoencephalopathy

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Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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