We measured the pulmonary venous concentration versus time outflow curves of 14C-diazepam (DM) and a vascular reference indicator (FITC-dextran) following the bolus injection of these indicators into the pulmonary artery of isolated rabbit lungs over the time course of granulamatous inflammation (GI) induced by i.v. injection of complete Freund's Adjuvant (CFA). The nonaquous, lipoid fraction of the lung tissue is accessible to DM, where it associates with different cellular constituents. We developed a kinetic model to quantify the DM-tissue interactions from the measured MID data. The model parameters are α, H and Kseq, where α and H are measures of the capacities of DM's rapidly and slowly (relative to capillary transit time) equilibrating interactions within the lung tissue in comparison to blood; and Kseq (ml/sec) is a measure of the sequestration rate of DM within the tissue. The values of these parameters correlate with the time course of the inflammatory response. These results indicate that, using the MID method, DM could be used to detect lung inflammation. Supported by Whitaker Foundation, Falk Trust, HL24349, and Dept Veterans Affairs. (Graph Presented).
|Original language||English (US)|
|State||Published - Mar 20 1998|
ASJC Scopus subject areas
- Molecular Biology