Detection of mip-like sequences and mip-related proteins within the family Rickettsiaceae

Nicholas P. Cianciotto*, William O'Connell, Gregory A. Dasch, Louis P. Mallavia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The Mip surface protein, a prokaryotic analog of the FK506-binding proteins, enhances the ability of Legionella pneumophila to infect macrophages and protozoa. Using mip-specific probes and low-stringency Southern hybridizations, we have detected DNA sequences homologous to mip within Coxiella burnetii and Rochalimaea quintana. Using specific anti-Mip antisera and immunoblot analysis, we also detected Mip-related proteins within these bacteria as well as within Rickettsia and Ehrlichia species. These data suggest that Mip-related proteins have broad significance for host-parasite interactions. However, they also indicate that care must be exercised when using mip probes or anti-Mip antibodies for the detection of Legionella organisms in water or clinical samples.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalCurrent Microbiology
Volume30
Issue number3
DOIs
StatePublished - Mar 1995

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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