Detection of murine cytomegalovirus (MCMV) DNA in skin using the polymerase chain reaction (PCR)

M. M. Abecassis*, X. Jiang, M. E. O’Neil, J. F. Bale

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We used the polymerase chain reaction (PCR) and primers for the immediate early gene of murine cytomegalovirus (MCMV) to detect MCMV DNA in skin harvested from mice during acute infection. MCMV DNA was also detected in DNA extracted from spleen and salivary gland of MCMV-infected mice, but not in the skin, salivary gland, or spleen of uninfected, seronegative mice. Detection of MCMV DNA in skin provides direct evidence that skin can serve as a vehicle for transmission of MCMV. This observation is relevant to humans, such as burn patients, who receive skin allografts that may be infected with cytomegalovirus.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalMicrobial Pathogenesis
Volume15
Issue number1
DOIs
StatePublished - Jul 1993

Keywords

  • Murine cytomegalovirus (MCMV)
  • Polymerase chain reaction (PCR)
  • Skin

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Detection of murine cytomegalovirus (MCMV) DNA in skin using the polymerase chain reaction (PCR)'. Together they form a unique fingerprint.

Cite this