Abstract
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).
| Original language | English (US) |
|---|---|
| Article number | 100372 |
| Journal | STAR Protocols |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 19 2021 |
Funding
This work was supported by grants RF1NS109157 and R01NS092823 from the NINDS/NIA awarded to J.R.M. The protocols were written by J.R.M. and I.S. J.R.M. has received consulting fees from SK Biopharmaceuticals, Neuron23, Sanofi-Genzyme, and Lysosomal Therapeutics. J.R.M. has ownership/investment interests in Lysosomal Therapeutics. This work was supported by grants RF1NS109157 and R01NS092823 from the NINDS / NIA awarded to J.R.M.
Keywords
- Molecular biology
- Neuroscience
- Protein biochemistry
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine
