Abstract
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).
Original language | English (US) |
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Article number | 100372 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 1 |
DOIs | |
State | Published - Mar 19 2021 |
Keywords
- Molecular biology
- Neuroscience
- Protein biochemistry
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Neuroscience(all)
- Immunology and Microbiology(all)
- Medicine(all)