Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue

Iva Stojkovska, Joseph R. Mazzulli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).

Original languageEnglish (US)
Article number100372
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

Keywords

  • Molecular biology
  • Neuroscience
  • Protein biochemistry

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine

Fingerprint

Dive into the research topics of 'Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue'. Together they form a unique fingerprint.

Cite this