Detection of prostaglandin H synthase (cyclooxygenase)-1 and -2 expression in single cancer cells by confocal Raman microspectroscopy

Kasem Nithipatikom*, Michael J. McCoy, Andre Kajdacsy-Balla, Sushma Kaul, Paul F. Lindholm, Sharon R. Hawi, Sunant Rochanakij, Fran Adar, William B. Campbell

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

Abstract

Prostaglandin H synthase (PGHS), commonly known as cyclooxygenase (COX), is the enzyme involved in the synthesis of biologically active prostanoids from arachidonic acid. There are two PGHS isozymes with identical functions, similar sizes and similar structures. PGHS-1 is constitutively expressed in most mammalian cells where as PGHS-2 is induced by various agents. PGHS-2 expression was increased with inflammation, mitogenesis, and some types of cancer. We have developed a method to simultaneously detect PGHS-1 and PGHS-2 in single cancer cells by using specific antibodies, surface-enhanced Raman scattering, and confocal Raman microspectroscopy. Cells were plated, cultured, incubated for immunocomplexation, and detected directly in the wells of a multiwell plate. The expression and localization of PGHS-1 and -2 in single malignant human hepatocytes was detected and compared to normal cells. Furthermore, the correlation between the expression of PGHS and the invasiveness of cancer cells was investigated in PC-3 cell sublines.

Original languageEnglish (US)
Pages (from-to)20-27
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3608
StatePublished - Jan 1 1999
EventProceedings of the 1999 Biomedical Applications of Raman Spectroscopy - San Jose, Ca, USA
Duration: Jan 25 1999Jan 26 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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