Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains

Mary A. Crane, Colleen Jue, Morgan Mitchell, Howard Lipton, Byung S. Kim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the λgt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.

Original languageEnglish (US)
Pages (from-to)173-186
Number of pages14
JournalJournal of Neuroimmunology
Volume27
Issue number2-3
DOIs
StatePublished - May 1990

Keywords

  • Antibody
  • Epitope
  • Fusion protein
  • Multiple sclerosis
  • Theiler's virus
  • λgt11

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains'. Together they form a unique fingerprint.

Cite this