Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains

Mary A. Crane; Colleen Jue; Morgan Mitchell; Howard Lipton; Byung S. Kim

doi:10.1016/0165-5728(90)90067-W

Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains

Mary A. Crane, Colleen Jue, Morgan Mitchell, Howard Lipton, Byung S. Kim^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the λgt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.

Original language	English (US)
Pages (from-to)	173-186
Number of pages	14
Journal	Journal of Neuroimmunology
Volume	27
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-5728(90)90067-W
State	Published - May 1990

Funding

Supported in part by National Multiple Sclerosis Society Grant RG2001-A2.

Keywords

Antibody
Epitope
Fusion protein
Multiple sclerosis
Theiler's virus
λgt11

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-5728(90)90067-W

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Crane, M. A., Jue, C., Mitchell, M., Lipton, H., & Kim, B. S. (1990). Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains. Journal of Neuroimmunology, 27(2-3), 173-186. https://doi.org/10.1016/0165-5728(90)90067-W

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title = "Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains",

abstract = "Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the λgt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.",

keywords = "Antibody, Epitope, Fusion protein, Multiple sclerosis, Theiler's virus, λgt11",

author = "Crane, {Mary A.} and Colleen Jue and Morgan Mitchell and Howard Lipton and Kim, {Byung S.}",

note = "Funding Information: Supported in part by National Multiple Sclerosis Society Grant RG2001-A2.",

year = "1990",

month = may,

doi = "10.1016/0165-5728(90)90067-W",

language = "English (US)",

volume = "27",

pages = "173--186",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

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}

Crane, MA, Jue, C, Mitchell, M, Lipton, H & Kim, BS 1990, 'Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains', Journal of Neuroimmunology, vol. 27, no. 2-3, pp. 173-186. https://doi.org/10.1016/0165-5728(90)90067-W

Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains. / Crane, Mary A.; Jue, Colleen; Mitchell, Morgan et al.
In: Journal of Neuroimmunology, Vol. 27, No. 2-3, 05.1990, p. 173-186.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system

T2 - differential patterns of antibody reactivity among different mouse strains

AU - Crane, Mary A.

AU - Jue, Colleen

AU - Mitchell, Morgan

AU - Lipton, Howard

AU - Kim, Byung S.

N1 - Funding Information: Supported in part by National Multiple Sclerosis Society Grant RG2001-A2.

PY - 1990/5

Y1 - 1990/5

N2 - Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the λgt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.

AB - Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the λgt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.

KW - Antibody

KW - Epitope

KW - Fusion protein

KW - Multiple sclerosis

KW - Theiler's virus

KW - λgt11

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Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the λgt11 system: differential patterns of antibody reactivity among different mouse strains

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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