Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers

Valentina S. Dimitrova; Saemee Song; Alexandra Karagiaridi; Anika Marand; Heather W. Pinkett

doi:10.1007/978-1-0716-2368-8_20

Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers

Valentina S. Dimitrova, Saemee Song, Alexandra Karagiaridi, Anika Marand, Heather W. Pinkett^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	375-387
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-0716-2368-8_20
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2507
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

This work was supported by NIH grants R01 AI139519 and R01 GM140584-01.

Keywords

ATP-binding cassette (ABC) transporter
Detergents
Diisobutylene-maleic acid (DIBMA) copolymer
Membrane proteins
Nanodiscs
Polymers
Purification
Solubilization
Styrene maleic acid (SMA) copolymer

ASJC Scopus subject areas

Genetics
Molecular Biology

Access to Document

10.1007/978-1-0716-2368-8_20

Cite this

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title = "Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers",

abstract = "The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.",

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doi = "10.1007/978-1-0716-2368-8_20",

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AU - Dimitrova, Valentina S.

AU - Song, Saemee

AU - Karagiaridi, Alexandra

AU - Marand, Anika

AU - Pinkett, Heather W.

PY - 2022

Y1 - 2022

N2 - The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.

AB - The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.

KW - ATP-binding cassette (ABC) transporter

KW - Detergents

KW - Diisobutylene-maleic acid (DIBMA) copolymer

KW - Membrane proteins

KW - Nanodiscs

KW - Polymers

KW - Purification

KW - Solubilization

KW - Styrene maleic acid (SMA) copolymer

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers

Abstract

Publication series

Funding

Keywords

ASJC Scopus subject areas

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